椎间盘退变中的Wnt信号通路  

Wnt signaling pathway in intervertebral disc degeneration

作　　者：范志鸿 张贤[2] 李超[1] Fan Zhihong;Zhang Xian;Li Chao(Nanjing University of Chinese Medicine,Wuxi 210023,Jiangsu Province,China;Wuxi Affiliated Hospital of Nanjing University of Chinese Medicine,Wuxi 214000,Jiangsu Province,China)

机构地区：[1]南京中医药大学,江苏省无锡市210023 [2]南京中医药大学无锡附属医院,江苏省无锡市214000

出　　处：《中国组织工程研究》2024年第12期1950-1955,共6页Chinese Journal of Tissue Engineering Research

基　　金：无锡市科技创新创业资金项目(Y2022009),项目负责人:张贤。

摘　　要：背景:Wnt信号通路在退变的椎间盘中过表达,而抑制其表达可延缓椎间盘退变进程,因此Wnt信号通路与椎间盘退变关系密切。目的:综述分析Wnt信号通路与椎间盘之间的相互关系,阐明Wnt信号通路在椎间盘退变进程中具体起到的作用和影响。方法:第一作者以“Intervertebral disc,Wnt,Cell proliferation,Cell senescence,Cell apoptosis,Extracellular matrix”为英文检索词,检索2000年至2023年1月PubMed数据库、Web of Science和OVID LWWspringlink数据库,查阅Wnt信号通路与椎间盘退变的相关研究文献,通过阅读整理保留54篇英文文献进行综述。结果与结论:①在胚胎的椎间盘形成过程中,Wnt信号通路可过表达,参与椎间盘形成并促进脊索后伸展。②在椎间盘退变过程中,Wnt信号通路可停滞细胞周期而抑制细胞增殖,使衰老相关蛋白表达增加并参与氧化应激而促进细胞衰老,此外还可受长链非编码RNA调控参与细胞凋亡。③Wnt信号通路亦可以减少细胞外基质相关蛋白合成、促进细胞外基质降解而加快椎间盘退变进程。④Wnt信号通路可通过作为早期椎间盘形成信号激活促进细胞再生,并参与诱导干细胞分化为椎间盘细胞而修复损伤的椎间盘,例如Wnt信号通路可诱导源自软骨终板细胞的干细胞向椎间盘迁移和转化。BACKGROUND:Wnt signaling pathway is overexpressed in degenerative intervertebral discs,and inhibition of its expression can delay the process of intervertebral disc degeneration.Therefore,Wnt signaling pathway is closely related to intervertebral disc degeneration.OBJECTIVE:To summarize the relationship between Wnt signaling pathway and intervertebral disc,especially the specific role and influence of Wnt signaling pathway in intervertebral disc degeneration.METHODS:The first author took“intervertebral disc,Wnt,cell proliferation,cell senescence,cell apoptosis,extracellular matrix”as the English search terms.PubMed,Web of science and OVID LWWSpringerlink were searched for articles published from 2000 to January 2023,and articles related to Wnt signaling pathway and disc degeneration were consulted.Totally 54 articles were reviewed by reading,collating and preserving.RESULTS AND CONCLUSION:(1)During intervertebral disc formation in embryos,Wnt signaling pathway is overexpressed,which is involved in intervertebral disc formation and promotes posterior extension of notochord.(2)During intervertebral disc degeneration,Wnt signaling pathway can inhibit cell proliferation by stagnating cell cycle,increase the expression of age-related proteins and promote cell aging by participating in oxidative stress,and participate in cell apoptosis by regulation of long non-coding RNA.(3)Wnt signaling pathway can also decrease extracellular matrix related protein synthesis,promote extracellular matrix degradation and accelerate intervertebral disc degeneration.(4)Wnt signaling pathway can promote cell regeneration by activating as early intervertebral disc formation signal and participate in inducing stem cells to differentiate into intervertebral disc cells to repair damaged intervertebral disc.For example,Wnt signaling pathway can induce stem cells from cartilage endplate cells to migrate and transform to intervertebral disc.

关 键 词：WNT信号通路 椎间盘退变 细胞增殖 细胞衰老 细胞凋亡 细胞外基质 长链非编码RNA 椎间盘修复 干细胞 中医药 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学] R364