Highly resilient and fatigue-resistant poly(4-methyl-ε-caprolactone)porous scaffold fabricated via thiol-yne photo-crosslinking/salt-templating for soft tissue regeneration  

作　　者：Zhaochuang Wang Wenhao Zhang Guo Bai Qiaohui Lu Xiaoyu Li Yan Zhou Chi Yang Yan Xiao Meidong Lang 

机构地区：[1]Shanghai Key Laboratory of Advanced Polymeric Materials,Key Laboratory for Ultrafine Materials of Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai,200237,PR China [2]State Key Laboratory of Bioreactor Engineering,School of Biotechnology,East China University of Science and Technology,Shanghai,200237,PR China [3]Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Department of Oral Surgery of Ninth People’s Hospital,College of Stomatology,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,PR China

出　　处：《Bioactive Materials》2023年第10期311-325,共15页生物活性材料（英文）

基　　金：support by the National Key Research and Development Program(2021YFB3800800);the National Natural Science Foundation of China(52273009);the National Natural Science Foundation of China(82271038);the Interdisciplinary Program of Shanghai JiaoTong University(YG2022QN050).

摘　　要：Elastomeric scaffolds, individually customized to mimic the structural and mechanical properties of natural tissues have been used for tissue regeneration. In this regard, polyester elastic scaffolds with tunable mechanical properties and exceptional biological properties have been reported to provide mechanical support and structural integrity for tissue repair. Herein, poly(4-methyl-ε-caprolactone) (PMCL) was first double-terminated by alkynylation (PMCL-DY) as a liquid precursor at room temperature. Subsequently, three-dimensional porous scaffolds with custom shapes were fabricated from PMCL-DY via thiol-yne photocrosslinking using a practical salt template method. By manipulating the Mn of the precursor, the modulus of compression of the scaffold was easily adjusted. As evidenced by the complete recovery from 90% compression, the rapid recovery rate of >500 mm min 1, the extremely low energy loss coefficient of <0.1, and the superior fatigue resistance, the PMCL20-DY porous scaffold was confirmed to harbor excellent elastic properties. In addition, the high resilience of the scaffold was confirmed to endow it with a minimally invasive application potential. In vitro testing revealed that the 3D porous scaffold was biocompatible with rat bone marrow stromal cells (BMSCs), inducing BMSCs to differentiate into chondrogenic cells. In addition, the elastic porous scaffold demonstrated good regenerative efficiency in a 12-week rabbit cartilage defect model. Thus, the novel polyester scaffold with adaptable mechanical properties may have extensive applications in soft tissue regeneration.

关 键 词：Elastomeric scaffold Thiol-yne photo-crosslinking Fatigue resistance Tissue regeneration 

分 类 号：R318[医药卫生—生物医学工程]