Multifunctional two-component in-situ hydrogel for esophageal submucosal dissection for mucosa uplift,postoperative wound closure and rapid healing  

作　　者：Xiong-Xin Lei Juan-Juan Hu Chen-Yu Zou Yan-Lin Jiang Long-Mei Zhao Xiu-Zhen Zhang Ya-Xing Li An-Ni Peng Yu-Ting Song Li-Ping Huang Jesse Li-Ling Hui-Qi Xie 

机构地区：[1]Department of Orthopedic Surgery and Orthopedic Research Institute,Laboratory of Stem Cell and Tissue Engineering,State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu,Sichuan,610041,China [2]Department of Otolaryngology,Head and Neck Surgery,West China Hospital,Sichuan University,Chengdu,Sichuan,610041,China [3]Institute of Biomedical Engineering and Informatics,Ilmenau University of Technology,Gustav-Kirchh Off Str.2,98693,Ilmenau,Germany [4]Department of Medical Genetics,West China Second Hospital,Sichuan University,Chengdu,Sichuan,610041,China

出　　处：《Bioactive Materials》2023年第9期461-473,共13页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(Grant No.32171351);the“1.3.5”Project for Disciplines of Excellence,West China Hospital,Sichuan University(Grant No.ZYJC18002);China Postdoctoral Science Foundation(2022M722277).

摘　　要：Endoscopic submucosal dissection (ESD) for gastrointestinal tumors and premalignant lesions needs submucosal fluid cushion (SFC) for mucosal uplift before dissection, and wound care including wound closure and rapid healing postoperatively. Current SFC materials as well as materials and/or methods for post-ESD wound care have single treatment effect and hold corresponding drawbacks, such as easy dispersion, short duration, weak hemostasis and insufficient repair function. Thus, designing materials that can serve as both SFC materials and wound care is highly desired, and remains a challenge. Herein, we report a two-component in-situ hydrogel prepared from maleimide-based oxidized sodium alginate and sulfhydryl carboxymethyl-chitosan, which gelated mainly based on "click" chemistry and Schiff base reaction. The hydrogels showed short gelation time, outstanding tissue adhesion, favorable hemostatic properties, and good biocompatibility. A rat subcutaneous ultrasound model confirmed the ability of suitable mucosal uplift height and durable maintenance time of AM solution. The in vivo/in vitro rabbit liver hemorrhage model demonstrated the effects of hydrogel in rapid hemostasis and prevention of delayed bleeding. The canine esophageal ESD model corroborated that the in-situ hydrogel provided good mucosal uplift and wound closure effects, and significantly accelerated wound healing with accelerating re-epithelization and ECM remodeling post-ESD. The two-component in-situ hydrogels exhibited great potential in gastrointestinal tract ESD.

关 键 词：HYDROGEL Endoscopic submucosal dissection Mucosa uplift Tissue sealant Wound healing 

分 类 号：O64[理学—物理化学]