Diversity of culturable alginate lyase-excreting bacteria associated with Sargassum  

作　　者：Xiaomeng Sun Zhao Xue Cui Chen Shoujin Fan Huihui Fu Peng Wang 

机构地区：[1]Life Science College,Shandong Normal University,Jinan 250014,China [2]College of Marine Life Sciences/Frontiers Science Center for Deep Ocean Multispheres and Earth System,Ocean University of China,Qingdao 266003,China

出　　处：《Acta Oceanologica Sinica》2023年第6期70-77,共8页海洋学报（英文版）

基　　金：The National Natural Science Foundation of China under contract Nos 32170127 and 42176156;the Fundamental Research Funds for the Central Universities under contract No.202141006。

摘　　要：Large numbers of bacteria live on the surface of various brown algae and can produce alginate lyases to consume alginate,an important component of the cell wall of brown algae.Sargassum is a genus of the largest canopyforming brown algae of more than 150 species,which are widely distributed in tropical and subtropical environments.However,our knowledge about the epiphytic bacteria and the alginate lyase-excreting bacteria from Sargassum is still primitive.Here,we investigated the diversity of the culturable epiphytic bacteria and alginate-degrading bacteria from Sargassum samples collected from the coastal seawaters of Shandong Province,China.In total,37 strains belonging to 21 genera in 3 phyla were isolated,including 15 previously unreported genera,of which Vibrio(6/37)and Pseudoalteromonas(5/37)are the dominant genera.Eight strains,mainly Vibrio and Pseudoalteromonas species,were further identified as alginate lyase-excreting strains that can utilize alginate for growth.The extracellular alginolytic activity of the 8 strains was determined,and strains Vibrio sp.C42and Pseudoalteromonas sp.M9 showed the highest activity.These results provide a better understanding of brown algae epiphytes and alginate-degrading bacteria,and are fundamental for further studies on the interactions between brown algae and their epiphytes.

关 键 词：SARGASSUM EPIPHYTE alginate lyase-excreting bacteria alginolytic activity 

分 类 号：Q948[生物学—植物学]