干扰NETO2通过调控JAK2/STAT3信号通路抑制肺腺癌细胞增殖和转移能力  

NETO2 inhibit the proliferation and metastasis ability of lung adenocarcinoma by regulated JAK2/STAT3 signaling pathways

作　　者：文礼[1] 李春姗[2] 李娇[1] 赵宁生 姚菲[2] WEN Li;LI Chunshan;LI Jiao;ZHAO Ningsheng;YAO Fei(Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi 530022,China;the Second Department of Tumor area,the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi 530022,China)

机构地区：[1]广西中医药大学第一附属医院呼吸与危重症医学科,广西南宁530022 [2]广西中医药大学第一附属医院仙葫院区肿瘤二区,广西南宁530022

出　　处：《国际检验医学杂志》2023年第14期1762-1767,共6页International Journal of Laboratory Medicine

摘　　要：目的研究神经纤毛及唾样蛋白2(NETO2)在肺腺癌中的表达及临床意义,并探讨NETO2对肺腺癌细胞增殖和转移能力的作用及其机制。方法采用免疫组化检测NETO2在肺腺癌组织中的表达水平,并分析NETO2的表达与肺腺癌患者临床病理参数及预后的关系。培养肺腺癌细胞A549,随机分为对照组(si-NC组)和实验组(si-NETO2-1组、si-NETO2-2组),分别转染NC siRNA和NETO2 siRNA-1、NETO2 siRNA-2,Western blot检测各组细胞中NETO2蛋白的表达水平;MTS实验和平板克隆实验检测各组细胞的增殖能力;Transwell实验检测各组细胞的转移能力;Western blot检测各组细胞中JAK2/STAT3信号通路蛋白JAK2、pJAK2、STAT3和pSTAT3蛋白表达。结果免疫组化结果显示,与癌旁组织相比,NETO2在肺腺癌组织中的表达增加(P<0.05),NETO2高表达与肺腺癌患者肿瘤T分期、淋巴结转移和TNM分期均相关(P<0.05),与NETO2低表达的肺腺癌患者相比,NETO2高表达的肺腺癌患者生存预后较差;与si-NC组相比,si-NETO2-1、si-NETO2-2组A549细胞中NETO2蛋白表达减少(P<0.05);与si-NC组相比,si-NETO2-1、si-NETO2-2组A549细胞的增殖和转移能力降低(P<0.05),A549组细胞中JAK2/STAT3信号通路相关蛋白pJAK2和pSTAT3蛋白表达降低(P<0.05),JAK2和STAT3蛋白表达无明显变化(P>0.05)。结论NETO2可能通过激活JAK2/STAT3信号通路促进肺腺癌的增殖和转移能力。Objective To study the expression and clinical significance of neuropilin and tolloid-like 2(NETO2),and discuss the role and mechanism of NETO2 on the proliferation and metastasis of lung adenocarcinoma cells.Methods Immunohistochemical was used to detect NETO2 expression level in lung adenocarcinoma tissue,and analyzed the relationship between NETO2 expression and clinical pathological parameters,prognosis of patients with lung adenocarcinoma.Cultivate lung adenocarcinoma cells A549,randomly divided into control group si-NC and experimental group si-NETO2-1,si-NETO2-2,and transmitted NC siRNA and NETO2 siRNA-1,NETO2 siRNA-2,Western blotting was used to detect the expression level of NETO2 protein in the group cells;MTS experimental and plate cloning experiments was used to detect the proliferation capabilities of each group cells;Transwell experiment was used to detect the metastasis of each group cells;Western blotting was used to detect the expression of JAK2/STAT3 signal pathway JAK2,pJAK2,STAT3 and pSTAT3 protein in each group of cells.Results Immunohistochemistry result showed that compared with the tissue of paracancer,the expression of NETO2 in the lung adenocarcinoma tissue was higher(P<0.05),the high expression of NETO2 is related to the tumor T,lymph nodes metastasis and TNM staging(P<0.05),compared to lung adenocarcinoma patients with NETO2 low-expressed,patients with NETO2 high-expressed have a poor prognosis;compared with the si-NC group,NETO2 protein expression in si-NETO2-1,si-NETO2-2 group A549 cells is reduced(P<0.05);compared with the si-NC group,the proliferation and metastasis capacity of si-NETO2-1,si-NETO2-2 groups A549 cells is reduced(P<0.05),JAK2/STAT3 signaling pathway related protein pJAK2 and pSTAT3 protein expression of si-NETO2-1,si-NETO2-2 groups A549 cells are decreased(P<0.05),JAK2 and STAT3 protein expression did not change significantly(P>0.05).Conclusion NETO2 may promote the proliferation and metastasis of lung adenocarcinoma by activating the JAK2/STAT3 signaling pathway.

关 键 词：肺腺癌 神经纤毛及唾样蛋白2 增殖 转移 JAK2/STAT3 

分 类 号：R446.8[医药卫生—诊断学]