麻杏石甘汤靶向AK1、CYP1B1、MGAM治疗儿童社区获得性肺炎  

Targeting AK1,CYP1B1,and MGAM with Maxing Shigan Decoction for Treatment of Community Acquired Pneumonia in Children

作　　者：职冬丽 刘欢 何巍 胡洋成 徐炎 ZHI Dongli;LIU Huan;HE Wei;HU Yangcheng;XU Yan(Xinyang Shangcheng County Hospital of Traditional Chinese Medicine,Henan Xinyang 465350,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China)

机构地区：[1]信阳市商城县中医院,河南信阳465350 [2]河南中医药大学第一附属医院,河南郑州450000

出　　处：《现代中医药》2023年第4期83-90,共8页Modern Chinese Medicine

基　　金：国家自然科学基金项目(82205190)。

摘　　要：目的通过儿童社区获得性肺炎转录组分析结合网络药理学及实验验证方法,阐述麻杏石甘汤治疗儿童社区获得性肺炎的主要靶点。方法使用R 3.5.0中limma、factoextra、dplyr、tibble、tidyr、ggplot2包进行分析及绘图,采用TCMSP、TCMID、PharmMapper数据库获取麻杏石甘汤成分及靶点。使用Elisa进行IL-6和TNF-α检测及Western Blot检测AK1、CYP1B1和MGAM。结果社区获得性肺炎组与对照组共有335个差异表达基因;麻杏石甘汤可减少A549细胞的炎症损伤;麻杏石甘汤可增加AK1的水平,减少CYP1B1和MGAM的水平,从而起到调控社区获得性肺炎的作用。结论麻杏石甘汤可调节社区获得性肺炎相关靶点及炎症水平。Objective To elucidate the main target of Maxing Shigan decoction in the treatment of children s community acquired pneumonia through Transcriptome analysis combined with network pharmacology and experimental verification methods.Methods R 3.5.0 were analyzed and plotted using limma,factoextra,dplyr,tible,tidyr,and ggplot2 packages.The components and targets of Maxing Shigan decoction were obtained using TCMSP,TCMID,and PharmaMapper databases.Using Elisa for IL-6 and TNF-αDetection and Western Blot detection of AK1,CYP1B1,and MGAM.Results There were 335 differentially expressed genes between the community acquired pneumonia group and the control group;Maxing Shigan decoction can reduce the inflammatory damage of A549 cells;Maxing Shigan decoction can increase the level of AK1,reduce the levels of CYP1B1 and MGAM,and thus play a role in regulating community acquired pneumonia.Conclusion Maxing Shigan decoction can regulate the targets and inflammation levels related to community acquired pneumonia.

关 键 词：麻杏石甘汤 治疗 社区获得性肺炎 网络药理学 转录组学 

分 类 号：R246.4[医药卫生—针灸推拿学]