普鲁士蓝纳米颗粒促进糖尿病皮肤创面愈合  

Prussian blue nanoparticles promote wound healing of diabetic skin

作　　者：贝颖 李文靖 李美运 苏梦 张津 黄玉 朱彦兆 李嘉丽 武艳[1] Bei Ying;Li Wenjing;Li Meiyun;Su Meng;Zhang Jin;Huang Yu;Zhu Yanzhao;Li Jiali;Wu Yan(College of Life Sciences,Mudanjiang Medical University,Mudanjiang 157000,Heilongjiang Province,China;Department of Chemical Engineering and Materials Science,Soochow University,Suzhou 215123,Jiangsu Province,China)

机构地区：[1]牡丹江医学院生命科学学院,黑龙江省牡丹江市157000 [2]苏州大学材料与化学化工学部,江苏省苏州市215123

出　　处：《中国组织工程研究》2024年第10期1526-1532,共7页Chinese Journal of Tissue Engineering Research

基　　金：黑龙江省自然科学基金联合引导项目(LH2020H076);项目负责人:武艳;牡丹江市指导性科技计划项目(HT2022JG125);项目负责人:武艳。

摘　　要：背景:炎症、氧化应激及细菌感染是糖尿病创面难愈合的主要原因,近年来各种无机纳米材料以其抗菌活性被广泛应用于皮肤创面愈合的治疗,但抗氧化和抗炎方面的作用有限。目的:考察普鲁士蓝纳米颗粒在抗氧化、抗炎和光热抗菌多方面的糖尿病创伤修复的效果。方法:制备普鲁士蓝纳米颗粒并进行表征。(1)体外实验:采用MTT法检测不同浓度普鲁士蓝纳米颗粒的生物相容性;在过氧化氢条件下,检测普鲁士蓝纳米颗粒的细胞保护作用及活性氧荧光表达;检测普鲁士蓝纳米颗粒分解过氧化氢和超氧阴离子自由基的能力;考察普鲁士蓝纳米颗粒抑制脂多糖诱导巨噬细胞炎症的作用;采用平板菌落计数法检测普鲁士蓝纳米颗粒的光热抗菌能力。(2)体内实验:腹腔注射链脲佐菌素建立糖尿病ICR小鼠模型,使用打孔器在背部建立直径6 mm全厚皮肤创面,分对照组(未给予治疗)、普鲁士蓝组及普鲁士蓝光照组干预,观察创面愈合与组织形态学变化。结果与结论:(1)体外实验:普鲁士蓝纳米颗粒在25-200μg/mL质量浓度下对细胞无毒性;普鲁士蓝纳米颗粒具有极强的抗氧化能力,能够抑制氧化应激条件下过度活性氧的产生及对细胞的杀伤,对过氧化氢有降解活性且具有很强的清除超氧阴离子自由基的能力;普鲁士蓝纳米颗粒还显示出显著的抗炎活性,并且在光照后显示出极强的抗菌能力。(2)体内实验:造模14 d后,普鲁士蓝组、普鲁士蓝光照组创面明显缩小,其中普鲁士蓝光照组创面愈合速度最快。苏木精-伊红和Masson染色显示,普鲁士蓝组、普鲁士蓝光照组创面可见大量的肉芽组织形成及胶原沉积,其中以普鲁士蓝光照组最多;免疫荧光染色显示,与对照组比较,普鲁士蓝组和普鲁士蓝光照组α-SMA和CD31表达明显增多(P<0.05),F4/80表达明显减少(P<0.05),其中以普鲁士蓝光照组改善更明显。(3)结BACKGROUND:Inflammation,oxidative stress and bacterial infection are the main causes of delayed wound healing in diabetes.In recent years,various inorganic nanomaterials have been widely used in the treatment of skin wound healing due to their antibacterial activities,but their effects on anti-oxidation and anti-inflammation are limited.OBJECTIVE:To investigate the effect of Prussian blue nanoparticles on the wound repair of diabetes in terms of antioxidant,anti-inflammatory and photothermal antibacterial activities.METHODS:Prussian blue nanoparticles were prepared and characterized.(1)In vitro:The biocompatibility of Prussian blue nanoparticles with different concentrations was detected by MTT assay.The cytoprotective effect of Prussian blue nanoparticles and the intracellular reactive oxidative species level were examined under the condition of hydrogen peroxide.The ability of Prussian blue nanoparticles to decompose hydrogen peroxide and superoxide anion radicals was tested;the effect of Prussian blue nanoparticles on lipopolysaccharide-induced macrophage inflammation was investigated.The photothermal antibacterial activity of Prussian blue nanoparticles was detected by the plate colony counting method.(2)In vivo:ICR mice were intraperitoneally injected with streptozotocin to establish a diabetes mouse model.After the model was successfully established,a 6 mm wound was created on the back with a hole punch.There were the control group(no treatment),the Prussian blue group and the Prussian blue with light group.The wound healing and histomorphological changes were observed.RESULTS AND CONCLUSION:(1)In vitro:Prussian blue nanoparticles in 25-200μg/mL were non-toxic to cells.Prussian blue nanoparticles had the extremely strong antioxidant capacity and mitigated the intracellular reactive oxidative species at a high oxidative stress environment,resulting in a pronounced cytoprotective effect.The Prussian blue nanoparticles not only exhibited hydrogen peroxide degradation activity but also showed strong superoxide

关 键 词：普鲁士蓝纳米颗粒 光热治疗 糖尿病小鼠模型 活性氧清除 巨噬细胞极化 创面愈合 

分 类 号：R459.9[医药卫生—治疗学] R319[医药卫生—临床医学] R364.3