3D打印多功能敷料治疗放创复合伤  

3D-printed multifunctional wound dressing for combined radiation and wound injury

作　　者：焦文成 代晶 严文锐 沈锦涛 胡静璐 金义光[2,4] 杜丽娜 Jiao Wencheng;Dai Jing;Yan Wenrui;Shen Jintao;Hu Jinglu;Jin Yiguang;Du Lina(Hebei University,Baoding 071002,Hebei Province,China;Beijing Institute of Radiation Medicine,Beijing 100850,China;General Hospital of Western War Zone,Chengdu 610083,Sichuan Province,China;Guangdong Pharmaceutical University,Guangzhou 510006,Guangdong Province,China;Henan University,Kaifeng 475004,Henan Province,China)

机构地区：[1]河北大学,河北省保定市071002 [2]中国人民解放军军事医学研究院辐射医学研究所,北京市100850 [3]中国人民解放军西部战区总医院,四川省成都市610083 [4]广东药科大学,广东省广州市510006 [5]河南大学,河南省开封市475004

出　　处：《中国组织工程研究》2024年第10期1562-1567,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:放创复合伤主要发生在肿瘤放疗和核辐射事故患者中,由于辐射破坏修复机制导致伤口愈合延迟或延长,目前仍缺乏有效的治疗策略。目的:针对放创复合伤的多种临床症状,制备抗菌、加速愈合和局部麻醉的多功能敷料。方法:以左氧氟沙星、丝素蛋白和盐酸利多卡因为原料,应用3D生物打印技术制备多功能创面敷料。①将多功能敷料分别放置于表面涂有金黄色葡萄球菌、大肠杆菌与铜绿假单胞菌的固定培养板上,37℃孵育过夜,检测抑菌圈直径。②将40只昆明种小鼠随机分单纯创伤组、放创模型组、治疗组、阳性药组,每组10只。放创模型组、治疗组、阳性药组小鼠全身接受60Coγ射线照射,辐射1 h后,在4组小鼠背部各制作1个直径1 cm的全层皮肤缺损创面,单纯创伤组、放创模型组创面涂抹生理盐水,阳性药组创面涂抹三乙醇胺乳膏,治疗组创面覆盖多功能敷料,每隔1 d更换敷料或换药,连续治疗14 d。创面造模后3,7,14 d,检测创面愈合率与血清白细胞介素6水平;创面造模后14 d,获取创面处皮肤组织,进行苏木精-伊红、Masson染色及细胞角蛋白14免疫组化染色。结果与结论:①3D打印多功能创面敷料具有良好的抗菌活性,对金黄色葡萄球菌、大肠杆菌与铜绿假单胞菌的抑菌圈直径分别为(4.15±0.09),(4.18±0.23),(4.35±0.13)cm。②随着造模时间的延长,小鼠创面逐渐愈合,治疗组、阳性药组造模后3,7,14 d的创面愈合率均高于放创模型组(P<0.01,P<0.001),并且治疗组创面愈合率高于阳性药组。随着造模时间的延长,小鼠血清白细胞介素水平呈先升高后降低的趋势,治疗组造模后3,7,14 d的血清白细胞介素水平均低于放创模型组。苏木精-伊红、Masson染色显示,单纯创伤组肉芽肿组织可见炎性细胞浸润,真皮层胶原纤维排列致密;放创模型组表皮层和真皮层正常结构被破坏,可见炎性细胞浸�BACKGROUND:Combined radiation and wound injury appeared mainly in patients with tumor radiotherapy and nuclear radiation accidents.The radiation destroys the repair mechanism,resulting in delayed or prolonged wound healing.It still lacks an effective therapeutic strategy currently.OBJECTIVE:To prepare multifunctional wound dressings based on the multiple clinical symptoms of combined radiation and wound injury,which are designed to be antibacteria,promoted healing and analgesics.METHODS:Using levofloxacin,fibroin and lidocaine hydrochloride as raw materials,3D bioprinting technology was applied to prepare the multifunctional wound dressing.(1)The multifunctional dressing was placed on a fixed culture plate coated with Staphylococcus aureus,Escherichia coli and Pseudomonas aeruginosa,and incubated at 37°C overnight to detect the diameter of the antibacterial zone.(2)40 Kunming mice were randomly divided into trauma group,radiation and trauma model group,treatment group and positive drug group,with 10 mice in each group.Mice in the radiation and trauma model group,treatment group and positive drug group were irradiated by 60Co gamma rays.After 1 hour of radiation,a full-layer skin defect wound with a diameter of 1 cm was made on the back of each mouse in the four groups.Normal saline was applied to the wounds of the trauma group and the radiation and trauma model group.Trethanolamine cream was applied to the wounds of the positive drug group.Multifunctional dressing was applied to the wounds of the treatment group.The dressing was changed every 2 days,and the treatment was continued for 14 days.Wound healing rate and serum interleukin-6 level were measured at 3,7 and 14 days after wound modeling.14 days after the wound modeling,the skin tissue of the wound was obtained and received hematoxylin-eosin staining,Masson staining and cytokeratin-14 immunohistochemical staining.RESULTS AND CONCLUSION:(1)3D-printed multifunctional wound dressing had good antibacterial activity.The antibacterial zone diameters against Stap

关 键 词：3D打印 放创复合伤 创面愈合 多功能敷料 敷料 丝素蛋白 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R944