大肠杆菌O157∶H7生物被膜状态下基因表达分析  

Gene Expression in Escherichia coli O157∶H7 Biofilms

作　　者：马蓝 彭晴[1] 徐小轻 杨硕 张宇微[1] 田丹丹 施琳波 石波[1] 乔宇[1] MA Lan;PENG Qing;XU Xiaoqing;YANG Shuo;ZHANG Yuwei;TIAN Dandan;SHI Linbo;SHI Bo;QIAO Yu(Feed Reasearch Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院饲料研究所,北京100081

出　　处：《中国农业科技导报》2023年第6期71-88,共18页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(32072773)。

摘　　要：生物被膜是细菌适应不良环境形成的一种特殊胞外结构,当细菌从浮游状态转换成生物被膜状态后其生理代谢活动也会随之发生改变。为了研究细菌形成生物被膜后生理代谢途径的变化及生物被膜形成的分子调控机制,以食源致病性大肠杆菌O157∶H7为试验对象,对其在浮游状态和生物被膜形成状态下的转录组进行测序分析。结果表明,与浮游状态相比,大肠杆菌O157∶H7在生物被膜形成状态有1652个显著差异表达的基因(上调821个,下调831个),其中鞭毛组装、细菌趋化性和双组分系统调控蛋白基因等在生物被膜形成阶段表达上调;ABC转运系统调控蛋白基因等表达下调。同时,碳代谢、氮代谢、脂肪酸代谢等多个代谢途径也发生了变化。上述结果为深入研究生物被膜形成的分子机制提供数据支持。Biofilm is a special extracellular structure of bacteria to adapt environmental stress.The physiological and metabolic activities of bacteria will change during the transition from the planktonic state to a biofilm.To investigate the changes of physiological metabolic pathways and the molecular regulation mechanism of biofilm formation,RNASeq technology was used in this study to examine the global gene expression profiles of Escherichia coli O157∶H7 during biofilm and planktonic growth states.The results showed that,in comparison to the planktonic growth state,there were 1652 differentially expressed genes in biofilm growth state of E.coli O157∶H7,including 821 upregulated genes and 831 downregulated genes.Genes involved in flagella assembly,bacterial chemotaxis in connection with bacterial motility,and bacterial two-component system regulatory system relating to environmental factors were up-regulated during E.coli O157∶H7 biofilm formation,while the genes involved in ABC transporters of nutrients and signal molecules were downregulated.In addition,changes in carbon metabolism,nitrogen metabolism,fatty acid metabolism and other metabolic pathways were found during E.coli O157∶H7 biofilm formation.Above results provided a scientific basis for the further study of biofilm formation mechanisms.

关 键 词：大肠杆菌 生物被膜 转录组 差异基因 富集分析 

分 类 号：R378.21[医药卫生—病原生物学]