抗生素对耐抽薹大白菜小孢子胚胎发生的影响  

The Effect of Antibiotics on Microspore Embryogenesis of Bolting Resistant Chinese Cabbage

作　　者：张梦璐 张红[3] 王超楠 黄志银 范伟强 张斌[2] ZHANG Menglu;ZHANG Hong;WANG Chaonan;HUANG Zhiyin;FAN Weiqiang;ZHANG Bin(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;State Key Laboratory of Vegetable Biobreeding,Tianjin Academy of Agricultural Sciences,Tianjin 300192,China;Tianjin Kerun Vegetable Research Institute,Tianjin Key Laboratory of Vegetable Genetics and breeding enterprises,Tianjin 300381,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]蔬菜生物育种全国重点实验室,天津市农业科学院,天津300192 [3]天津科润蔬菜研究所,天津市蔬菜遗传育种企业重点实验室,天津300381

出　　处：《中国蔬菜》2023年第7期45-51,共7页China Vegetables

基　　金：现代农业产业技术体系专项资金资助项目(CARS-23-G-05);天津市(蔬菜)现代农业产业技术体系创新团队项目(ITTVRS2021003);天津市农业科学院财政种业创新研究项目(2022ZYCX007)。

摘　　要：为克服耐抽薹大白菜游离小孢子培养中出胚难的问题,以23个耐抽薹大白菜品种为试材,探究不同基因型间小孢子胚胎发生的差异以及两性霉素-B、灰黄霉素对小孢子胚诱导的影响。结果表明:采用NLN-13培养基,23份耐抽薹大白菜材料中仅有3份诱导出胚,胚诱导成功率为13.0%,且不同基因型间小孢子出胚率存在显著差异,表明基因型是影响大白菜小孢子胚胎发生的重要因素。添加适宜浓度的两性霉素-B和灰黄霉素,23份耐抽薹大白菜材料的胚诱导成功率分别达到60.9%和82.6%,添加1.0~2.0 mg·L^(-1)两性霉素-B和0.5 mg·L^(-1)灰黄霉素时诱导出胚材料数量最多,胚诱导率最高可达0.467胚·蕾^(-1)和0.733胚·蕾^(-1),表明培养基中加入抗生素能够在一定程度上促进耐抽薹大白菜小孢子胚胎形成。In order to overcome the problem of difficult embryo formation in isolated microspore culture of bolting resistant Chinese cabbage,23 bolting resistant Chinese cabbage samples with difficult embryogenesis were used as experimental materials to explore the differences of microspore embryogenesis among different genotypes and the effects of amphotericin-B and griseofulvin on microspore embryo induction.The results showed that only 3 materials of 23 bolting resistant Chinese cabbage were produced embryos by NLN-13 medium,and the success rate of embryo induction was 13.0%,and there were significant differences in microspore embryo formation among different genotypes,indicating that genotype is an important factor affecting microspore embryogenesis in Chinese cabbage.By adding appropriate concentrations of amphotericin-B and griseofulvin,the success rate of embryo induction of 23 bolting resistant Chinese cabbage materials reached 60.9%and 82.6%,respectively,and the maximum number of embryonic materials was induced when 1.0-2.0 mg·L^(-1) amphotericin-B and 0.5 mg·L^(-1) griseofulvin were added.The highest embryo induction rate was 0.467 embryos per bud and 0.733 embryos per bud,indicating that adding antibiotics in the medium could promote the formation of microspore embryo in bolting resistant Chinese cabbage to a certain extent.

关 键 词：耐抽薹大白菜 小孢子培养 基因型 两性霉素-B 灰黄霉素 

分 类 号：S634.1[农业科学—蔬菜学]