小儿柴桂退热口服液对发热模型大鼠的退热作用和机制研究  

Antipyretic Effect and Mechanism of Xiaoer Chaigui Tuire Oral Liquid on Fever Model Rats

作　　者：洪倩 马增春 王增慧 韩莉 王青 杨阳 HONG Qian;MA Zengchun;WANG Zenghui;HAN Li;WANG Qing;YANG Yang(The Affiliated Huaihai Hospital of Xuzhou Medical University/The 71st Group Army Hospital of CPLA Army,Xuzhou 221004,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)

机构地区：[1]徐州医科大学附属淮海医院/解放军陆军第七十一集团军医院,徐州221004 [2]军事科学院军事医学研究院辐射医学研究所,北京100850

出　　处：《世界中医药》2023年第11期1558-1562,共5页World Chinese Medicine

基　　金：国家中医药管理局国家中医药传承创新团队项目(ZYYCXTD-C-202009)。

摘　　要：目的:研究小儿柴桂退热口服液对酵母发热模型大鼠的退热作用机制。方法:将45只雄性SD大鼠皮下注射20%酵母混悬液,制备酵母发热模型大鼠,通过随机数字表随机分为模型组、布洛芬组、柴桂低剂量组、柴桂中剂量组、柴桂高剂量组,每组9只,模型组给予0.75 mL/kg剂量生理盐水溶液灌胃,布洛芬组给予0.75 mL/kg剂量右旋布洛芬口服混悬液灌胃,柴桂各剂量组分别给予1.25 mL/kg、2.5 mL/kg、5 mL/kg剂量小儿柴桂退热口服液灌胃;另取9只未造模大鼠为空白组,给予0.75 mL/kg剂量生理盐水溶液灌胃。给药1 h、2 h后,比较各组大鼠体温变化情况;给药2 h后,比较各组大鼠血清白细胞介素-1β(IL-1β)、一氧化氮(NO)、髓过氧化物酶(MPO)含量及下丘脑前列腺素E_(2)(PGE_(2))、环磷酸腺苷(cAMP)含量。结果:与模型组比较,治疗1 h、2 h后各给药组大鼠体温升高幅度均有所降低,且随着时间延长体温下降趋势越显著。与模型组比较,布洛芬组、柴桂各剂量组大鼠IL-1β、NO、MOP含量以及PGE_(2)、cAMP含量均显著降低(均P<0.01);柴桂各剂量组的效应强度与剂量均正相关,但均弱于布洛芬组。结论:小儿柴桂退热口服液能够降低酵母发热模型大鼠的体温升高,且退热作用较为温和;其作用机制与抑制血清炎症介质IL-1β、NO、MOP的生成与释放,减少下丘脑发热中枢PGE_(2)、cAMP的合成与释放有关。Objective:To study the antipyretic effect and mechanism of Xiaoer Chaigui Tuire Oral Liquid(XECGTR)on yeast-induced fever model rats.Methods:Forty-five male SD rats were subcutaneously injected with a 20%yeast suspension to establish the yeast-induced fever model.The model rats were randomly divided into a model group,an ibuprofen group,and low-,medium-,and high-dose XECGTR groups,with nine rats in each group.The model group received normal saline orally at 0.75 mL/kg,the ibuprofen group received D-ibuprofen oral suspension at 0.75 mL/kg,and the XECGTR groups received XECGTR orally at 1.25,2.5,and 5 mL/kg,respectively.Another nine rats without modeling were assigned to the blank group and received normal saline orally at 0.75 mL/kg.The changes in body temperature were compared among the groups at 1 and 2 h after administration.Two hours after administration,the levels of interleukin-1β(IL-1β),nitric oxide(NO),myeloperoxidase(MPO),prostaglandin E_(2)(PGE_(2)),and cyclic adenosine monophosphate(cAMP)in the serum were compared among the groups.Results:The temperature increase in each treatment group was reduced at 1 and 2 h after administration,and the temperature decrease became more significant over time as compared with that in the model group.Compared with the results in the model group,the levels of IL-1β,NO,MPO,PGE_(2),and cAMP in the ibuprofen group and XECGTR groups significantly decreased(all P<0.01).The efficacy of XECGTR was positively correlated with the dosage,but weaker than that of the ibuprofen group.Conclusion:XECGTR can reduce the increase in body temperature in yeast-induced fever model rats,and its antipyretic effect is relatively mild.The mechanism may be related to the inhibition of the production and release of serum inflammatory mediators IL-1β,NO,and MPO,as well as the reduction of the synthesis and release of PGE_(2) and cAMP in the hypothalamic fever center.

关 键 词：小儿柴桂退热口服液 退热作用 退热机制 发热模型大鼠 酵母 体温 炎症介质 下丘脑发热中枢 

分 类 号：R285.5[医药卫生—中药学]