响应面法优化内蒙古肉苁蓉多糖提取工艺  

Optimization of Polysaccharide Extraction Process of Cistanches Herba Produced in Inner Mongolia by Response Surface Methodology

作　　者：霍达 张竞男 马微 张善林 扈瑞平 张俊锋 马春丽 HUO Da;ZHANG Jingnan;MA Wei;ZHANG Shanlin;HU Ruiping;ZHANG Junfeng;MA Chunli(Basic Medical College,Inner Mongolia Medical University,Hohhot 010110,China;Inner Mongolia Cistanche Industrial Co.,LTD,Hohhot 010020,China;Inner Mongolia Kunming Cigarette Co.LTD,Hohhot 010030,China)

机构地区：[1]内蒙古医科大学基础医学院,呼和浩特010110 [2]内蒙古苁蓉实业有限责任公司,呼和浩特010020 [3]内蒙古昆明卷烟有限责任公司,呼和浩特010030

出　　处：《世界中医药》2023年第12期1667-1671,共5页World Chinese Medicine

基　　金：内蒙古自治区科技计划项目(2019GG160);第“十一批”草原英才工程,内蒙古道地蒙药材免疫活性多糖制备及其构效关系研究创新人才团队项目(20210787);内蒙古自治区蒙医药协同创新项目(MYYXT201901)。

摘　　要：目的:确定热水浸提法提取内蒙古肉苁蓉多糖的最优条件。方法:选取热水浸提法提取内蒙古肉苁蓉多糖,把内蒙古肉苁蓉作为原材料,以内蒙古肉苁蓉多糖提取率为响应值,通过单因素实验找到最优单因素条件,再将单因素实验结果利用响应面法对内蒙古肉苁蓉多糖的提取条件进行优化。结果:实验结果表明,热水浸提法的最优条件是:液料比为45∶1(mL∶g)、热水浸提温度为100℃,时间为130 min、乙醇醇沉浓度为100%。在此条件下,内蒙古肉苁蓉的多糖提取率为11.207%,与响应面法预测值11.609%非常接近。结论:通过实验证明采用响应面法对内蒙古肉苁蓉多糖提取工艺得到的优化条件合理可靠,可为下一步工业化生产提供可靠的依据。Objective:To optimize the conditions for extracting polysaccharides from Cistanches Herba produced in Inner Mongolia with hot water.Methods:With the yield of polysaccharides extracted from Cistanche Herba as the response value,single factor tests were carried out to identify the significant influencing factors,which were then optimized by response surface methodology.Results:The results showed that the yield of polysaccharides was the highest(11.207%)when the extraction was conducted with the liquid-to-solid ratio at 45∶1(mL∶g)at 100℃for 130 min and the ethanol concentration of 100%for precipitation.The yield obtained under these conditions was close to the predicted value of 11.609%by response surface methodology.Conclusion:It is proved by experiments that the optimal conditions for extracting polysaccharides from Cistanches Herba produced in Inner Mongolia by response surface methodology are reasonable and reliable,which can provide a reliable basis for the industrial production.

关 键 词：内蒙古肉苁蓉 热水浸提法 多糖 提取率 响应面法 液料比 温度 时间 醇沉浓度 

分 类 号：R284[医药卫生—中药学]