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作 者:唐娜 宋莉 张秉华 王嫦鹤 TANG Na;SONG Li;ZHANG Binghua;WANG Changhe(Shaanxi Institute for Food and Drug Control,Shaanxi,Xi'an 710065,China)
机构地区:[1]陕西省食品药品检验研究院,陕西西安710065
出 处:《中国医药科学》2023年第13期77-79,103,共4页China Medicine And Pharmacy
摘 要:目的建立高效液相色谱法测定复方胰酶片中猪去氧胆酸和鹅去氧胆酸的含量。方法采用SunShell HFC_(18~30)色谱柱(100mm×4.6mm,2.6μm),流动相为乙腈-水-磷酸(35∶65∶0.1),检测波长为192nm,柱温为40℃,流速1.0 ml/min。结果猪去氧胆酸和鹅去氧胆酸在所考察的浓度范围内的峰面积与浓度呈良好的线性关系,相关系数分别为0.9999和0.9998;平均加样回收率分别为76.6%[相对标准偏差(RSD)为4.5%]和91.4%(RSD为1.9%)。结论此方法准确、灵敏,可用于复方胰酶片中猪去氧胆酸和鹅去氧胆酸的测定。Objective To establish a high-performance liquid chromatography(HPLC)method for the determination of hyodeoxycholic acid and chenodeoxycholic acid in compound pancreatin tablets.Methods SunShell HFC_(18-30) column(100 mm×4.6 mm,2.6μm)was adopted.The mobile phase was acetonitrile-water-phosphoric acid(35∶65∶0.1),the detection wavelength was 192 nm,the column temperature was 40℃,and the flow rate was 1.0 ml/min.Results There was a good linear correlation between the peak area and the concentration of hyodeoxycholic acid and chenodeoxycholic acid in the investigated concentration range,and the correlation coefficients were 0.9999 and 0.9998 respectively.The average recovery rates of sample addition were 76.6%(RSD was 4.5%)and 91.4%(RSD was 1.9%),respectively.Conclusion This method is accurate and sensitive,and it can be used for the determination of hyodeoxycholic acid and chenodeoxycholic acid in compound pancreatin tablets.
关 键 词:复方胰酶片 猪去氧胆酸 鹅去氧胆酸 高效液相色谱法
分 类 号:R917[医药卫生—药物分析学]
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