复方柏连制剂对小鼠抗炎镇痛作用及湿热型UC模型大鼠TNF-α、IL-6、IL-1β表达的影响  

Anti-inflammatory and Analgesic Effects of Compound Bailian Preparation in Mice and the Expressions of TNF-α,IL-6 and IL-1β in Damp-heat Ulcerative Colitis Model Rats

作　　者：郭媛媛 朱志坤 王飘 王五兵 李思琪 陈凌云 余晓玲[2] GUO Yuanyuan;ZHU Zhikun;WANG Piao;WANG Wubing;LI Siqi;CHEN Lingyun;YU Xiaoling(Pharmaceutical Laboratory,College of Traditional Chinese Medicine,Yunnan University of Traditional Chinese Medicine,Kunming 650500,China;Pharmacy Department of Kunming Traditional Chinese Medicine Hospital,Kunming 650500,China)

机构地区：[1]云南中医药大学中药学院药剂实验室,昆明650500 [2]昆明市中医医院药剂科,昆明650500

出　　处：《医药导报》2023年第8期1104-1109,共6页Herald of Medicine

基　　金：昆明市中药(民族药)医院制剂研发科技创新团队(No.2018-1-R-17245);昆明市医院制剂质量控制模式创新中心(No.2019-1-N-25318000002943)。

摘　　要：目的探讨复方柏连灌肠剂和凝胶剂的急性毒性、抗炎镇痛和药效学作用,为临床安全用药提供思考方案。方法给小鼠以最大浓度24 h内重复2次灌肠,给大鼠以折算浓度灌肠7 d,分别观察其毒性反应和死亡情况;建立小鼠二甲苯致耳肿胀模型、冰醋酸致扭体模型、环境因素加三硝基苯磺酸(TNBS)/乙醇法致大鼠溃疡性结肠炎(UC)模型,分别观察复方柏连灌肠剂和凝胶剂药效学作用。结果复方柏连制剂灌肠最大给药量生药为161.6 g·kg^(-1),相当于人每日用量的96倍;复方柏连灌肠剂和凝胶剂大、中、小剂量组均能显著降低小鼠耳肿胀率,减少扭体次数,改善湿热型UC模型大鼠结肠组织的病理损伤及降低TNF-α、IL-6、IL-1β含量(P<0.05)。结论复方柏连灌肠剂和凝胶剂临床拟用剂量安全且对直肠黏膜没有刺激性,具有一定的抗炎、镇痛、治疗UC的作用。Objective To study the acute toxicity,anti-inflammatory and analgesic effects and preliminary pharmacodynamics of compound bailian enema and gel to provide consideration for clinically safe drug use.Methods Mice were given the maximum concentration of enema twice within 24 hours,rats were given the equivalent concentration of enema for seven days,and the toxicity and death were observed,respectively;the mouse model of xylene-induced ear swelling and glacial acetic acid-induced writhing were established.Model,environmental factors plus trinitrobenzene sulfonic acid(TNBS)/ethanol method induced ulcerative colitis(UC)model was established in rats,respectively,to observe the pharmacodynamic effects of compound bailian enema and gel was observed on those models.Results The maximum dose of compound bailian preparation for enema was 161.6 g of crude drug·kg^(-1),equivalent to 96 times the daily dose of human beings;Compound baitian enema and gel at high,medium,and low doses can significantly reduce the ear swelling rate of mice,reduce the times of body twisting,improve the pathological damage of colon tissue in rats with damp heat UC model and reduce TNF-α、IL-6、IL-1β activity(P<0.05).Conclusion The clinical doses of the compound bailian enema and gel are safe and no irritating effect to the rectal mucosa and have certain anti-inflammatory,analgesic,and therapeutic effects on UC rats.

关 键 词：复方柏连制剂 溃疡型结肠炎 急性毒性 黏膜刺激性 抗炎作用 镇痛作用 

分 类 号：R971[医药卫生—药品]