钻地风三萜类化学成分及含量测定研究  

Study on Chemical Composition and Content Determination of Triterpenoids from Rubus ellipticus Smith.var obcordatus(Franch.)Focke

作　　者：王飘 李思琪 王五兵 李昌芬 张意雪 易志恒 陈凌云 WANG Piao;LI Siqi;WANG Wubing;LI Changfen;ZHANG Yixue;YI Zhiheng;CHEN Lingyun(School of Chinese Materia Medica,Yunnan University of Chinese Medicine,Kunming 650500,China;Key Laboratory of External Drug Delivery System and Preparation Technology Research in Universities of Yunnan Province,Kunming 650500,China;Sailing Pharmaceutical Technology Group Co.,Ltd,Yuxi 653100,China)

机构地区：[1]云南中医药大学中药学院,昆明650500 [2]云南省高校外用给药系统与制剂技术研究重点实验室,昆明650500 [3]赛灵药业科技集团股份有限公司,云南玉溪653100

出　　处：《医药导报》2023年第8期1199-1203,共5页Herald of Medicine

基　　金：云南省重大科技专项基金资助项目(202102AA310029)。

摘　　要：目的研究钻地风[Rubus ellipticus Smith.var obcordatus(Franch.)Focke]的化学成分,并进行含量测定。方法采用柱色谱技术对钻地风进行分离纯化,根据波谱数据鉴定其结构,通过高效液相色谱(HPLC)法对各成分进行含量测定。结果分离得到6个三萜类化合物单体,分别鉴定为蔷薇酸(1)、刺梨苷(2)、苦莓苷(3)、rotundic acid-28-O-β-D-glucopyranosyl ester(4)、rosamultin(5)、2α,3β,23-trihydroxyurs-12,18-dien-28-oic acid 28-O-β-D-glucopyranoside(6)。建立的HPLC色谱条件为以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,检测波长203 nm,柱温25℃。结论化合物4为首次从钻地风中分离得到;所建立的5个成分同时测定方法稳定可靠,蔷薇酸、刺梨苷和苦莓苷在钻地风中含量较高,可考虑作为钻地风的质控指标,为其资源利用提供依据。Objective To study the chemical composition of Rubus ellipticus Smith.var obcordatus(Franch.)Focke and to determine its content.Methods The separation and purification of Rubus ellipticus Smith.var obcordatus(Franch.)Focke was performed by column chromatography,and its structure was identified based on the spectral data,and the content of each component was determined by high performance liquid chromatography(HPLC).Results Six triterpenoid monomers were isolated and identified as euscaphic acid(1),kaji-ichigoside F1(2),niga-ichigoside F1(3),rotundic acid-28-O-β-D-glucopyranosyl ester(4),rosamultin(5),2α,3β,23-trihydroxyurs-12,18-dien-28-oic acid 28-O-β-D-glucopyranoside(6).The established HPLC chromatographic conditions were gradient elution with acetonitrile-0.1%phosphoric acid solution as the mobile phase,detection wavelength of 203 nm,and column temperature 25℃.Conclusion Compound 4 was first isolated from Rubus ellipticus Smith.var obcordatus(Franch.)Focke;the established method for the simultaneous determination of five components was stable and reliable,and euscaphic acid,kaji-ichigoside F1 and niga-ichigoside F1 were high as the key contents,which can be considered as quality control indicators for Rubus ellipticus Smith.var obcordatus(Franch.)Focke.The study provides a basis for its resource utilization.

关 键 词：钻地风 化学分离 五环三萜 含量测定 

分 类 号：R979[医药卫生—药品]