木鳖子苷Ⅰ的NMR测定研究  

Study on NMR determination of momordica saponin Ⅰ

作　　者：王嘉祺 吴子薇 时晓颖 李晓波[1] 王梦月[1] WANG Jiaqi;WU Ziwei;SHI Xiaoying;LI Xiaobo;WANG Mengyue(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《上海中医药大学学报》2023年第3期42-47,101,共7页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(82274061)。

摘　　要：目的:研究木鳖子苷Ⅰ的化学结构及纯度测定方法。方法:采用2D NMR技术、水解方法确定化学结构及NMR数据归属;采用1H-NMR方法测定纯度。结果:运用2D NMR技术,结合酸水解、碱水解方法,快速确定了化学结构,首次完成了木鳖子苷ⅠNMR数据全归属。建立了1H-NMR方法测定木鳖子苷Ⅰ的纯度,该方法不需要特殊对照品,操作简单、快速准确。结论:本研究为复杂皂苷类成分的结构及纯度测定提供了方法借鉴。Objective:To investigate the chemical structure and purity determination method of momordica saponinⅠ(MSⅠ).Methods:2D NMR technology and hydrolysis method were used to determine the chemical structure and assign the NMR data.1H-NMR technology was used to determine the purity.Results:The chemical structure of MSⅠwas rapidly determined and the NMR data was completely assigned for the first time by 2D NMR technology combined with acid and alkaline hydrolysis.A simple,rapid,and accurate method for the purity determination of MSⅠwas established by 1H-NMR which didn’t need specific reference substance.Conclusion:The study provided a methodological reference for the chemical structure and purity determination of complex saponins.

关 键 词：木鳖子苷Ⅰ 木鳖子 NMR 信号归属 纯度测定 

分 类 号：R284.1[医药卫生—中药学]