归脾汤对慢性应激抑郁大鼠前额叶皮质神经元可塑性的影响  

Effect of Guipi Decoction on plasticity of prefrontal cortex neurons in rats with chronic stress-induced depression

作　　者：李婷婷[1,2] 李祥婷 陈羽 吴婷 张静思 蔡定芳[1,2] LI Tingting;LI Xiangting;CHEN Yu;WU Ting;ZHANG Jingsi;CAI Dingfang(Department of Encephalopathy,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Institute of Neurology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Integrative Medicine,Zhongshan Hospital,Fudan University,Shanghai 200032,China)

机构地区：[1]上海中医药大学附属曙光医院脑病科,上海201203 [2]上海中医药大学附属曙光医院神经病学研究所,上海201203 [3]复旦大学附属中山医院中西医结合科,上海200032

出　　处：《上海中医药大学学报》2023年第3期57-62,共6页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海中医药大学附属曙光医院国家自然科学基金青年孵育项目(SGKJ-201923)。

摘　　要：目的:观察归脾汤对慢性不可预见性温和应激(CUMS)抑郁大鼠前额叶皮质神经元可塑性的影响,探讨归脾汤抗抑郁的作用机制。方法:100只健康雄性SD大鼠进行糖水适应,建立糖水偏好基线后,选取符合要求的大鼠随机分为对照组(15只)和造模组(75只)。对照组大鼠常规饲养,造模组大鼠采用CUMS结合孤养3周建立抑郁模型。将造模组大鼠随机分为模型组、氟西汀组及归脾汤高、中、低剂量组,分别给予纯水、氟西汀及相应剂量归脾汤灌胃。给药4周后,对各组大鼠进行行为学检测,并用免疫荧光法和Western blot法观察各组大鼠前额叶皮质神经元核(NeuN)和糖原合成酶激酶-3β(GSK-3β)表达水平。结果:免疫荧光和Western blot结果显示,归脾汤高剂量组大鼠前额叶皮质NeuN表达较模型组显著增多(P<0.05,P<0.01);Western blot结果显示,归脾汤高剂量组大鼠前额叶皮质GSK-3β表达较模型组显著减少(P<0.01)。结论:归脾汤可能通过调节抑郁大鼠前额叶皮质神经元的可塑性发挥抗抑郁作用。Objective:To observe the effect of Guipi Decoction on the plasticity of prefrontal cortex neurons in rats with chronic unpredictable mild stress(CUMS)-induced depression,and explore the antidepressant mechanism of Guipi Decoction.Method:Ninety healthy male SD rats were subjected to sugar water adaptation,and after establishing the baseline of sugar water preference,they were randomly divided into control group(15 rats)and modeling group(75 rats).Rats in the control group received routine feeding,while rats in the modeling group was subjected to CUMS combined with solitary housing for 3 weeks to establish a depression model.Rats in the modeling group were randomly divided into model group,fluoxetine group,and high,medium,and low dose Guipi Decoction groups,and were administered with pure water,fluoxetine,and corresponding doses of Guipi Decoction by gavage,respectively.After 4 weeks of administration,behavioral tests were conducted on rats in each group,and the expression levels of neuronal nuclei(NeuN)and glycogen synthase kinase-3β(GSK-3β)in the prefrontal cortex of rats in each group were observed using immunofluorescence and Western blot methods.Result:Immunofluorescence and Western blot results showed that the expression of NeuN in the prefrontal cortex of rats in the high dose Guipi Decoction group was significantly higher than that of the model group(P<0.05,P<0.01);Western blot results showed that the expression of GSK-3βin the prefrontal cortex of rats in the high dose Guipi Decoction group was significantly lower than that of the model group(P<0.01).Conclusion:Guipi Decoction may exert antidepressant effect by regulating the plasticity of prefrontal cortex neurons in depressed rats.

关 键 词：归脾汤 抑郁 前额叶皮质 慢性不可预见性温和应激 神经元可塑性 

分 类 号：R285.5[医药卫生—中药学]