小于胎龄儿肠道菌群的前瞻性研究  

Prospective study of the gut microbiota in small-for-gestational-age infants

作　　者：徐慧松 胡万建 王青兰 蔡晨 XU Hui-song;HU Wan-jian;WANG Qing-lan;CAI Chen(Neonatology Department,Linping District Maternal and Child Health Hospital of Hangzhou City,Hangzhou,Zhejiang 310000,China;不详)

机构地区：[1]杭州市临平区妇幼保健院新生儿科,浙江310000

出　　处：《中国微生态学杂志》2023年第5期516-521,共6页Chinese Journal of Microecology

基　　金：2021年杭州市卫生科技计划一般(B类)项目(B20210358)。

摘　　要：目的调查小于胎龄(small for gestational age,SGA)儿出生早期肠道菌群特征,探究肠道菌群丰度对婴儿发育的影响。方法选择2021年7月至2022年2月在杭州市临平区妇幼保健院产科孕检母亲40例、出生婴幼儿40例作为研究对象,将分娩SGA婴儿妊娠妇女与分娩适于胎龄(appropriate for gestational age,AGA)婴儿妊娠妇女1∶1进行配对,匹配指标包括是否足月、BMI值是否在正常范围、母亲年龄±5岁以及顺产,作病例对照研究。符合入组标准的产妇40例,其中分娩SGA婴儿母亲20例,为SGA_M组;分娩AGA婴儿母亲20例,为Control_M组。对应新生儿40例,其中SGA婴儿20例,为SGA_C组;AGA婴儿20例,为Control_C组。采用高通量16S rRNA基因测序技术明确SGA婴儿肠道菌群的定植情况,采用Illumina NovaSeq 6000测序系统对V3~V4可变区进行高通量测序,获取肠道菌群的丰度和多样性分布情况。结果SGA组婴儿肠道内大肠埃希菌、双歧杆菌、肠球菌及乳杆菌拷贝数量均显著低于对照组(均P<0.05);基于alpha多样性Chao1指数的分析,估计群落中包含物种的数目,可以看出对照组与SGA组之间物种数目差异极显著;基于beta多样性主坐标分析PCoA的Unweighted_uniFrac_PCoA图,对照组和SGA组之间物种丰度有差异,ANOSIM,R=0.5,P<0.01。在科水平中,Lactobacillaceae、Enterobacteriaceae及Bifidobacteriaceae在4个组中差异均有统计学意义(均P<0.05)。在LEfSe分析中,与SGA组比较,对照组中差异显著的优势菌群数量较少,仅g_Leuconnostoc、f_Lactobacillaceae及g_Dongia为显著上调差异菌;而在SGA组中,差异上调菌群为g_Rothia、f_Micrococcaceae及o_Micrococcales,这些菌同样在SGA_M组与Control_M组比较中为SGA_M组显著上调的差异菌。结论SGA婴幼儿肠道菌群的丰富度和多样性个体间变异度高,致病菌占优,易导致患儿出现肠道微生态系统失衡,其与败血症等SGA婴幼儿并发症具有密切相关性。Objective To investigate the characteristics of gut microbiota in small-for-gestational-age(SGA)infants and explore the effect of gut microbiota abundance on infant development.Methods A total of 40 cases of mothers and 40 cases of infants were selected from July 2021 to February 2022 in the maternity department of Linping District Maternal and Child Health Hospital as subjects of this study.The pregnant women who gave birth to SGA babies and pregnant women who gave birth to AGA babies were matched 1∶1,and the matching indicators included whether they were full term,whether the BMI value was in the normal range,the mother's age was±5 years old and the birth was normal,and a casecontrol study was conducted.Among them,20 cases of mothers gave birth to children smaller than gestational age,which was SGA_M group.Twenty cases of mothers of gestational age infants were in Control_M group;Twenty children were small for gestational age(SGA_C group).High-throughput 16S rRNA gene sequencing technology was used to determine the colonization of the intestinal faecal tract of SGA infants.Illumina NovaSeq 6000 sequencing system was used to con-duct high-throughput sequencing of the V3-V4 variable region,and the abundance and diversity distribution of intestinal flora were obtained.Results The copy numbers of Escherichia coli,Bifidobacterium,Enterococcus and Lactobacillus in the intestinal tract of infants in the SGA group were lower than those in the control group(all P<0.05).Based on the analysis ofα-diversity Chao1 index,the number of species included in the community was estimated.It could be seen from the figure that the difference in the number of species between Control-C and SGA-C was extremely significant.The unweighted_uniFrac_PCoA graph of PCoA was analyzed based on the principal coordinates ofβ-diversity.Species abundance was different between Control-C and SGA-C,ANOSIM,R=0.5,P<0.01.At the family level,Lactobacillaceae,Enterobacteriaceae and Bifidobacteriaceae were significantly different among the four groups(

关 键 词：小于胎龄儿 肠道菌群 菌群丰度 高通量测序 生长发育 

分 类 号：R378[医药卫生—病原生物学]