孕期BMI变化与新生儿胎便菌群和免疫状况的关系研究  

Gestational weight gain correlated with neonatal microbiota and immunity

作　　者：杨晋 衣薪燕 卢姗 王娇 宋庆宇 文姝 YANG Jin;YI Xin-yan;LU Shan;WANG Jiao;SONG Qing-yu;WEN Shu(Department of Microecology,Dalian Medical University,Dalian,Liaoning 116044,China;Department of Central Laboratory,Dalian Municipal Central Hospital,Dalian,Liaoning 116033,China;不详)

机构地区：[1]大连医科大学基础医学院微生态学教研室,辽宁大连116044 [2]大连市中心医院中心实验室 [3]大连市中心医院妇产科 [4]大连市中心医院检验科

出　　处：《中国微生态学杂志》2023年第5期522-531,共10页Chinese Journal of Microecology

摘　　要：目的探究妊娠妇女孕期BMI变化对新生儿肠道菌群定植和免疫发育的影响。方法选取孕期BMI增幅<6 kg/m^(2)的12人为Am组,孕期BMI增幅≥6 kg/m^(2)的12人为Bm组。Am组妊娠妇女对应的新生儿为Af组,Bm组妊娠妇女对应的新生儿为Bf组。收集妊娠妇女孕晚期血清、新生儿脐带血清、孕晚期粪便以及新生儿出生第一个24 h胎便。对妊娠妇女的粪便及对应新生儿胎便进行DNA提取,采用16S rRNA基因测序方法分析菌群变化。利用生物信息学方法分析妊娠妇女及对应新生儿不同分组菌群结构变化。利用酶联免疫吸附法(ELISA)检测妊娠妇女孕晚期血清、脐带血清中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平,比较组间变化。结果BMI增幅≥6 kg/m^(2)(Bm组)的妊娠妇女与BMI增幅<6 kg/m^(2)(Am组)的相比,肠道菌群alpha多样性存在降低趋势,beta多样性存在差异。Bm组妊娠妇女粪便样本中脱硫弧菌属显著降低,拟杆菌门、纲、目、科、属以及韦荣球菌科、属显著升高。Bf组与Af组相比,胎便菌群alpha多样性同样存在降低趋势,beta多样性同样存在差异。Bf组变形菌门、γ-变形菌纲、β-变形菌纲显著升高,拟杆菌门等显著降低。Bm组较Am组血清中性粒细胞与淋巴细胞比值(neutrophil-lymphocyte ratio,NLR)存在差异,TNF-α、IL-6水平有升高趋势。在对应的新生儿的脐带血清中Bf组TNF-α、IL-6水平显著升高。结论孕期BMI增幅≥6 kg/m^(2)的妊娠妇女肠道菌群和新生儿胎便菌群存在紊乱。孕期BMI增幅≥6 kg/m^(2)的妊娠妇女孕晚期血清中和新生儿脐带血清中炎症因子增高。妊娠妇女孕期BMI增幅与拟杆菌门及拟杆菌属的增加相关,能够影响妊娠妇女和新生儿的肠道菌群与免疫。Objective To observe the effects of maternal BMI changes during pregnancy on neonatal microbiota and immune development.Methods A total of 24 pairs of qualified pregnant women and their corresponding newborns were screened for the study,12 of the women with a gestational BMI increase of<6 kg/m^(2)were divided into Am Group,with their newborns into Af group,while the other 12 with a gestational BMI increase of≥6 kg/m^(2)were in Bm Group,with their newborns into Bf group.Serum and feces of the third trimester of pregnancy,and umbilical cord serum and stool at 24 hours after birth were collected respectively from the women and newborns.DNA was extracted from the feces for 16S rRNA gene sequencing to observe the changes of flora.Bioinformatics methods was used to explore diverse bacterial taxa.TNF-αand IL-6 in serum and umbilical cord serum were detected with enzyme-linked immunosorbent assay(ELISA),and compared between groups.Results Comparison between Bm Group and Am Group,as well as between Bf and Af group,showed a decrease trend in alpha diversity of intestinal flora,and there was a significant difference in beta diversity.LEfSe analysis showed a significant increase in Bacteroides and Veillonella,and significant decrease in Vibrio desulfurizae in Bm group.In Bf group,Proteobacteria increased significantly,while Bacteroidota and Firmicutes decreased significantly.There was a difference in neutrophil-lymphocyte ratio between Bm group and Am group,and TNF-αand IL-6 showed an increasing trend in Bm group.In the corresponding neonatal umbilical cord blood serum,there were significant increases in TNF-αand IL-6 in the Bf group.Conclusion The gut microbiota of pregnant women with gestational BMI gain of≥6 kg/m^(2)and stool microbiota of their newborns were disturbed.The inflammation markers in the serum of pregnant women with gestational BMI gain of≥6 kg/m^(2)in the third trimester of pregnancy and in umbilical cord blood of their neonatals were elevated.The gestational BMI gain was associated with the increas

关 键 词：孕期BMI增幅 肠道菌群 新生儿免疫 妊娠妇女菌群免疫 

分 类 号：R378.12[医药卫生—病原生物学]