杉木根系接种AMF染色条件的筛选及观察  

Screening and Observation of AMF Staining Conditions for Chinese Fir Roots Inoculation

作　　者：许静静 田云龙 魏博 马祥庆[1,2] 吴鹏飞 XU Jing-jing;TIAN Yun-long;WEI Bo;MA Xiang-qing;WU Peng-fei(Forestry College,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Fujian Engineering Research Center of Chinese Fir Germplasm Enhancement,Fuzhou 350002,Fujian,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建省杉木种质创新工程研究中心,福建福州350002

出　　处：《西北林学院学报》2023年第4期166-172,共7页Journal of Northwest Forestry University

基　　金：福建省自然科学基金重点项目(2020J02029);福州市科学技术局科技计划项目(2021-P-035)。

摘　　要：丛枝菌根真菌(AMF)侵染情况观察是深入研究植物根系与土壤微生物共生机制的重要基础。以杉木同一无性系1年生幼苗为研究对象,在接种根内球囊霉(Glomus intraradices,Gi)3个月后,基于经典的台盼蓝染色法,利用正交试验设计,对影响杉木根系染色效果的KOH浓度、温度以及染色时间等主要因素进行优化研究,比较分析杉木不同根序的AMF侵染率差异性规律。结果表明,对于杉木幼苗1级根,筛选出20%的KOH浓度、水煮温度90℃和染色时间30 min这一组合的染色效果较好,显微制片中的根系皮层组织细胞、菌丝结构清晰;对于杉木2级根,采用20%KOH浓度、水煮温度96℃和染色时间10 min处理下的染色效果较好;相对木质化程度最高的3级根,采用20%KOH浓度、水煮温度100℃、染色时间30 min或10%KOH浓度、水温96℃、染色时间20 min这2种组合的染色效果较好。与杉木幼苗1级根和3级根相比,2级根的AMF侵染率明显较高(P<0.05)。The observation of arbuscular mycorrhizal fungi(AMF)infection is an important basis for the further study of the symbiotic mechanisms between plant root system and soil microorganisms.In this study,objected with annual seedlings cultivated from the same Chinese fir clone,using an orthogonal test design based on the classical Taipan Blue staining method,the main factors of the staining effect in Chinese fir root system tissues,including KOH concentration,boiling water temperature and staining time were optimized respectively,after the tested seedlings were inoculated with Glomus intraradices(Gi)for three months.Furthermore,the differences of AMF infection rate among different root orders were compared and analyzed.Main results showed that the combination conditions with 20%KOH concentration,water temperature of 90℃and staining time of 30 min were better for the first order roots of Chinese fir seedlings,and both of the cellular and mycelial structures of the root cortex in the microfilm were clear.For the second order of Chinese fir roots,the staining effect was better under the treatment with 20%KOH concentration,water temperature of 96℃and staining time of 10 min.Both of the two combination conditions of 20%KOH concentration,water temperature of 100℃and staining time of 30 min or 10%KOH concentration,water temperature of 96℃and staining time of 20 min were more effective in staining the tertiary roots with the highest relative lignification.Compared with the first or third order of Chinese fir roots,AMF infestation rate in the second order root was significantly higher(P<0.05).

关 键 词：杉木 根级 丛枝菌根真菌 染色方法 侵染率 

分 类 号：S718.8[农业科学—林学]