锌胁迫下外生菌根真菌对巨桉生长的影响  

Effects of Ectomycorrhizal Fungi on the Growth of Eucalyptus grandis Under Zinc Stress

作　　者：王新阳 刘紫怡 任颖 唐明 WANG Xin-yang;LIU Zi-yi;REN Ying;TANG Ming(Guangdong Laboratory for Lingnan Modern Agriculture,College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510640,Guangdong,China)

机构地区：[1]岭南现代农业科学与技术广东省实验室华南农业大学林学与风景园林学院,广东广州510640

出　　处：《西北林学院学报》2023年第4期180-188,共9页Journal of Northwest Forestry University

基　　金：广州市科技计划重点项目(201904020022);岭南现代农业科学与技术广东省实验室科研项目(NZ2021025);国家自然科学基金(32071639)。

摘　　要：以巨桉为试验材料,接种外生菌根真菌(Ectomycorrhizal fungi,ECMF)彩色豆马勃(Pisolithus tinctorius 21118),通过构建彩色豆马勃-巨桉共生体系,不同浓度锌胁处理30 d后对巨桉株高、生物量、根系形态特征、叶绿素含量和叶片N含量进行评估。结果表明,低浓度Zn(200μmol·L^(-1))促进巨桉生长、根系的发育以及叶片营养元素氮(N)和叶绿素的积累,较高浓度(400μmol·L^(-1))和高浓度(600μmol·L^(-1))Zn则产生抑制作用;巨桉和彩色豆马勃具有较强的共生能力,接种彩色豆马勃显著改善了巨桉在Zn处理下的生长,促进了巨桉叶片的N累积,增强了巨桉光合作用;较高浓度和高浓度Zn胁迫下,接种彩色豆马勃能够促进巨桉生物量的积累,改善植株根系形态,增加根表面积、根体积、根尖数,促进细根的形成,提高根系对Zn的耐受性。研究结果为菌根技术在重金属污染土壤植被恢复的应用提供了理论依据。Eucalyptus grandis was used as material,in which Ectomycorrhizal fungi(Pisolithus tinctorius 21118)were inoculated to establish the symbiont system.Then,the established symbionts were subject to Zinc stress.After 30 days of zinc treatment,the seedling height,biomass,root morphological characteristics,chlorophyll content and leaf nitrogen contents of E.grandis were measured and evaluated to examine the effects of ectomycorrhizal fungi on the growth of E.grandis under Zinc stress.The results showed that low concentration of Zn(200μmol·L^(-1))promoted the growth,root development,nitrogen(N)and chlorophyll accumulation in the leaves of E.grandis,while high concentration of Zn(400 or 600μmol·L^(-1))presented inhibited effects.Strong symbiont effect was observed between E.grandis and P.tinctorius.And under the Zn treatment,inoculation of P.tinctorius significantly improved the growth,promoted the leaves N accumulation,and enhanced the photosynthesis of E.grandis.For E.grandis under higher and the highest concentration of Zn stress,inoculation of P.tinctorius could promote the accumulation of biomass,improve root morphology,increase root surface area,root volume and number of root tips,promote the formation of fine roots,and improve root tolerance to Zn.This study provides a theoretical basis for mycorrhizal vegetation restoration in heavy metal-contaminated soils.

关 键 词：外生菌根真菌 巨桉 锌胁迫 生长量 

分 类 号：S718.81[农业科学—林学]