左归丸对早发性卵巢功能不全肾阴虚证大鼠的影响  

Effects of Zuogui Pills on kidney-yin deficiency syndrome of premature ovarian insufficiency rats

作　　者：曹莹[1] 任宇航[1] 陈玥 王佩娟[2] Cao Ying;Ren Yuhang;Chen Yue;Wang Peijuan(Department of Gynecology,Suzhou Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine,Suzhou 215009,China;Department of Obstetrics and Gynecology,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210000,China)

机构地区：[1]南京中医药大学附属苏州市中医医院妇科,苏州215009 [2]南京中医药大学附属中西医结合医院妇产科,南京210000

出　　处：《国际中医中药杂志》2023年第7期847-851,共5页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81903998);江苏省自然科学基金重大发展项目(BE2019766);江苏省研究生科研与实践创新计划项目(SJCX21-0754)。

摘　　要：目的研究左归丸对早发性卵巢功能不全肾阴虚证大鼠的影响。方法将40只SD雌性未交配大鼠按随机数字表法分为空白组、模型组、左归丸组、戊酸雌二醇组,每组10只。除空白组外,其余各组大鼠均皮下注射透明带3(ZP3)和灌胃左旋甲状腺素钠复制早发性卵巢功能不全肾阴虚证模型;于造模同时左归丸组和戊酸雌二醇组大鼠灌胃相应药物,空白组和模型组大鼠灌胃等体积生理盐水,1次/d,连续21 d。于实验第0、7、14、21天测定大鼠体重、耳温,计算卵巢指数、子宫指数、甲状腺指数,采用ELISA法检测血清中环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、皮质醇、FSH、LH、雌二醇(E2)水平,并计算cAMP/cGMP比值。HE染色观察卵巢病理形态学改变。结果实验第14、21天,与模型组比较,左归丸组大鼠体重升高(P<0.05),耳温降低(P<0.05);与模型组比较,左归丸组大鼠卵巢指数、子宫指数、甲状腺指数降低(P<0.05),血清cAMP/cGMP、皮质醇、FSH、LH水平降低(P<0.05),E2水平升高(P<0.05)。结论左归丸对左甲状腺素钠片联合ZP3致早发性卵巢功能不全肾阴虚证大鼠有一定的改善作用。Objective To study the effects of Zuogui Pills on rats with kidney-yin deficiency syndrome of premature ovarian insufficiency.Methods Totally 40 SD female unmated rats were randomly divided into blank group,model group,Zuogui Pills group and Bujiale group,with 10 rats in each group.Except for blank group,rats in other groups were subcutaneously injected with ZP3 and gavaged with levothyroxine sodium to induce kidney-yin deficiency syndrome model of premature ovarian insufficiency.At the same time of modeling,Zuogui Pills group and Bujiale group received corresponding drugs for gavage,and the other groups received corresponding solvent for gavage,once a day,for consecutive 21 days.On day 0,7,14 and 21,ear temperature and body weight of rats were measured,and the ovarian index,uterus index and thyroid index were calculated.Serum levels of adenosine cyclic phosphate(cAMP),cyclic guanosine phosphate(cGMP),Cortisol,follicle-stimulating hormone(FSH),luteinizing hormone(LH)and estradiol(E2)were detected by ELISA.Pathological changes of ovary was observed with HE staining.Results On day 14 and 21,compared with model group,the body weight of rats in Zuogui Pills group increased(P<0.05),and the ear temperature decreased(P<0.05);compared with model group,the ovarian index,uterine index and thyroid index of rats in Zuogui Pills group decreased(P<0.05),the levels of serum cAMP/cGMP,cortisol,FSH and LH decreased(P<0.05),and the level of E2 increased(P<0.05).Conclusion Zuogui Pills have certain improvement effect on rats with kidney-yin deficiency syndrome induced by levothyroxine sodium tablets combined with ZP3.

关 键 词：左归丸 早发性卵巢功能不全 肾阴虚 大鼠 左甲状腺素钠片 

分 类 号：R285.5[医药卫生—中药学]