纤维素临氢水解反应产物的高效液相色谱分析  

Analysis of Hydrohydrolysis Products of Cellulose by High Performance Liquid Chromatography

作　　者：农韦健 陈小鹏[3] 余金方 韦雪娟 徐艺玮 梁家琪 梁杰珍[3] 

机构地区：[1]中国科技开发院广西分院,广西南宁530022 [2]广西科开成林科技有限公司,广西南宁.530000 [3]广西大学化学化工学院,广西南宁530004 [4]广西科技项目评估中心有限公司,广西南宁530022

出　　处：《大众科技》2023年第5期37-39,107,共4页Popular Science & Technology

基　　金：南宁市培养新世纪学术和技术带头人专项资金资助项目(No.2020010)。

摘　　要：采用高效液相色谱法测定纤维素临氢水解反应产物,建立了定量分析的方法和回归方程。考察了不同流动相组成、流速、精密度和回收率对分析效果的影响。结果表明,较适宜的分析条件为流动相乙腈∶水为3∶1,流速为1 mL/min。在此分析条件下,纤维素临氢水解反应产物的峰面积与主要成分乙二醇、1,2-丙二醇和丙三醇标准品的线性关系良好,相关系数在0.99以上,精密度实验中乙二醇、1,2-丙二醇和丙三醇的RSD值在1.2%~1.9%之间,重复性实验乙二醇、1,2-丙二醇和丙三醇的RSD值在1.4%~1.6%之间,加标回收率实验的回收率在98.44%~102.55%之间,所建立的方法比较可靠。A quantitative analysis method and regression equation were established for the determination of cellulose hydrohydrolysis products using high-performance liquid chromatography.The effects of different mobile phase compositions,flow rates,precision,and recovery rates on the analysis results were investigated.The results showed that the optimal analysis conditions were mobile phase acetonitrile 3:1 in water,and a flow rate of 1 mL/min.Under these analytical conditions,the peak area of cellulose hydrohydrolysis products had a good linear relationship with the main components of ethylene glycol,1,2-propylene glycol and propylene glycol standard products,and the correlation coefficient was above 0.99.The RSDS of ethylene glycol,1,2-propylene glycol and propylene glycol in precision experiment were between 1.2%~1.9%.RSDS of ethylene glycol,1,2-propylene glycol and glycerol were in the range of 1.4%~1.6%,and the recoveries of standardized tests were in the range of 98.44%~102.55%.The established method was more reliable.

关 键 词：纤维素 临氢水解 高效液相色谱 

分 类 号：O657.72[理学—分析化学]