不同来源接种物对不同原料产氢烷潜力的影响及微生物群落结构分析  

Investigation of Hydrogen and Methane Productions Potential for Different Raw Materials and Analysis of Microbial Community Structure for Different Substrates Inocula

作　　者：李凯[1] 赵紊骁赟 刘思雨 张欣蕾 李禄恩 梅宝帆 井琳琳 孙雅雅 袁海荣[1] LI Kai;ZHAO Wenxiaoyun;LIU Siyu;ZHANG Xinlei;LI Luen;MEI Baofan;JING Linlin;SUN Yaya;YUAN Hairong(State Key Laboratory of Chemical Resource Engineering,Department of Environmental Science and Engineering,Beijing University of Chemical Technology,Beijing 100029,China)

机构地区：[1]北京化工大学化工资源有效利用国家重点实验室,环境科学与工程系,北京100029

出　　处：《中国沼气》2023年第4期31-39,共9页China Biogas

基　　金：国家重点研发计划项目(2019YFC1904104)。

摘　　要：接种物的特性关系到能否正常启动厌氧消化系统,是厌氧消化系统启动成败的关键所在。以牛粪(NF)、秸秆厌氧消化液(ZY)和餐厨干发酵物出料(CN)3种接种物为研究对象,研究了不同接种物对不同原料产氢烷潜力的影响及其微生物群落结构特性。结果表明:厨余-CN和玉米秸秆-NF组的最高产甲烷和产氢速率分别为42.4和26.5 mL·g^(-1)VS·d^(-1)。玉米秸秆-NF组的累积甲烷产量最高为80.9 mL·g^(-1)VS,分别比其它实验组累积产甲烷量高出18.1%~255.4%。玉米秸秆-NF组在产甲烷和产氢气方面均为最优,其次是厨余-CN组。Proteiniphilum(16.2%)、Turicibacter(12.6%)和Methanocorpusculum(80.5%)、Methanobrevibacter(18.6%)是NF接种物在属水平上的优势细菌和古菌。因此,不同微生物样本的微生物群落结构存在较大显著性差异,不同原料的产氢烷潜力均受不同接种物中的多种微生物种类和丰度的影响,牛粪接种物消化玉米秸秆原料的产氢产甲烷潜力最优。The characteristics of the inoculum are related to the normal start of the anaerobic digestion(AD)system,which is the key of the success or failure of AD startup.In this study,three kinds inocula of cow manure(NF),liquid fraction of straw digestate(ZY)and residues of food waste AD(CN)were used.The effect of different substrates inocula on hydrogen and methane productions potential of different substrates were investigated and microbial community structure was analyzed.The results showed that the highest methane production and hydrogen production rates of the kitchen waste-CN and corn stover-NF groups were 42.4 and 26.5 mL·g^(-1) VS·d^(-1),respectively.The highest cumulative methane yield for corn straw-NF group was 80.9 mL·g^(-1) VS,which was 18.1%~255.4%higher than that of the other experimental groups.The corn stover-NF group was optimal in methane production and hydrogen production,followed by the kitchen waste-CN group.Proteiniphilum(16.2%),Turicibacter(12.6%),Fermentimonas(10.4%),Lysinibacillus(9.0%)and Methanocorpusculum(80.5%),Methanobrevibacter(18.6%)are the predominant bacteria and archaea for the NF inoculum at the genus level.Therefore,there were significant differences for the microbial community structure of different microbial samples.The hydrogen and methane production potential of different substrates were influenced by multiple microbial species and abundances in different inocula.The hydrogen and methane production potential were optimal for corn stover-NF groups.

关 键 词：接种物 微生物 厌氧消化 群落结构 

分 类 号：X705[环境科学与工程—环境工程] X712