过敏性哮喘患儿血清lncRNA MEG3表达与Th17/Treg平衡的关系  

Relationship between serum lncRNA MEG3 expression and Th17/Treg balance in children with allergic asthma

作　　者：王星 李瑞婷 马小苗 WANG Xing;LI Ruiting;MA Xiaomiao(Department of Laboratory Medicine,the First Affiliated Hospital of Xi’an Jiaotong University,Shaanxi Province,Xi’an 710089,China;Department of Pediatrics,the First Affiliated Hospital of Xi’an Jiaotong University,Shaanxi Province,Xi’an 710089,China;Department of Pediatrics,Ankang Maternal and Child Health Hospital,Shaanxi Province,Ankang 725000,China)

机构地区：[1]西安交通大学第一附属医院检验科,陕西西安710089 [2]西安交通大学第一附属医院儿科,陕西西安710089 [3]陕西省安康市妇幼保健院儿科,陕西安康725000

出　　处：《中国医药导报》2023年第20期113-116,共4页China Medical Herald

基　　金：陕西省卫生健康科研项目(2018F004)。

摘　　要：目的 探讨过敏性哮喘患儿外周血长链非编码核糖核酸母系表达的基因3(lncRNA MEG3)表达与辅助性T细胞(Th)17/调节性T细胞(Treg)失衡的关系。方法 选择2018年1月至2022年1月西安交通大学第一附属医院(以下简称“我院”)收治的75例过敏性哮喘患儿为哮喘组,另选择我院儿童保健门诊的49名健康儿童为对照组。检测两组Th17细胞百分比、Treg细胞百分比、Th17/Treg比值,lncRNA MEG3表达、白细胞介素(IL)-17、IL-22、IL-10、转化生长因子-β(TGF-β)水平及第1秒用力呼气容积(FEV1),计算FEV1与用力肺活量比值(FEV1/FVC)、FEV1占预计值百分比(FEV1%pred)。分析lncRNA MEG3表达与Th17/Treg及其细胞因子、肺功能的相关性。结果 哮喘组lncRNA MEG3表达、Treg细胞百分比低于对照组,Th17细胞百分比、Th17/Treg高于对照组(P<0.05)。哮喘组IL-17、IL-22水平高于对照组,TGF-β、IL-10水平低于对照组(P<0.05)。哮喘组FEV1、FEV1/FVC、FEV1%pred低于对照组(P<0.05)。哮喘组患儿lncRNA MEG3表达与Treg细胞百分比、IL-10、TGF-β、FEV1、FEV1/FVC、FEV1%pred呈正相关(r=0.502、0.415、0.491、0.493、0.428、0.497,P<0.05),与Th17细胞百分比、Th17/Treg、IL-17、IL-22呈负相关(r=-0.501、-0.727、-0.631、-0.452,P<0.05)。结论 过敏性哮喘患者外周血lncRNA MEG3表达降低,且与Th17/Treg失衡及肺功能低下有关。Objective To investigate the relationship between maternal long chain non-coding ribonucleic acid maternally expressed gene 3(lncRNA MEG3)expression and T helper cell(Th)17/regulatory T cell(Treg)imbalance in children with allergic asthma.Methods A total of 75 children with allergic asthma admitted to the First Affiliated Hospital of Xi’an Jiaotong University(hereinafter referred to as“our hospital”)from January 2018 to January 2022 were selected as asthma group,and 49 healthy children from the Children’s health clinic of our hospital were selected as control group.The percentage of Th17 cells,the percentage of Treg cells,Th17/Treg ratio,lncRNA MEG3 expression,interleukin(IL)-17,IL-22,IL-10,transforming growth factor-β(TGF-β)levels,and forced expiratory volume in one second(FEV1)were detected,the ratio of FEV1 to forced vital capacity(FEV1/FVC),and the percentage of FEV1 to predicted value(FEV1%pred)were calculated.The correlation between lncRNA MEG3 expression and Th17/Treg and its cytokines,and lung function were analyzed.Results The expression of lncRNA MEG3,the percentage of Treg cells in asthma group were lower than those in control group,while the percentage of Th17 cells,Th17/Treg were higher than those in control group(P<0.05).The levels of IL-17 and IL-22 in asthma group were higher than those in control group,while the levels of TGF-βand IL-10 were lower than those in control group(P<0.05).The FEV1,FEV1/FVC,and FEV1%pred in asthma group were lower than those of control group(P<0.05).The expression of lncRNA MEG3 in children with asthma was positively correlated with the percentage of Treg cells,IL-10,TGF-β,FEV1,FEV1/FVC,and FEV1%pred(r=0.502,0.415,0.491,0.493,0.428,0.497,P<0.05),and the percentage of Th17 cells,Th17/Treg,IL-17,and IL-22 were negatively correlated(r=-0.501,-0.727,-0.631,-0.452,P<0.05).Conclusion The expression of lncRNA MEG3 in peripheral blood of patients with allergic asthma is decreased,which is related to Th17/Treg imbalance and low lung function.

关 键 词：过敏性哮喘 长链非编码核糖核酸 母系表达的基因3 免疫 辅助性T细胞17 调节性T细胞 

分 类 号：R562[医药卫生—呼吸系统]