玉米脂氧合酶家族基因的鉴定及禾谷镰刀菌侵染与茉莉酸甲酯处理下的表达分析  

Identification of Lipoxygenase Family Genes in Maize(Zea mays)and Expression Analysis Under Fusarium graminearum Infection and MeJA Treatment

作　　者：李薇[1] 张玮煜 刘英姿 马文骁 李紫媛 张康 邢继红[1] 曹宏哲 董金皋[1] LIWei;ZHANGWei-Yu;LIU Ying-Zi;MAWen-Xiao;LI Zi-Yuan;ZHANG Kang;XING Ji-Hong;CAO Hong-Zhe;DONG Jin-Gao(State Key Laboratory of North China Crop Improvement and Regulation/Hebei Key Laboratory of Plant Physiology and Molecular Pathology,Hebei Agricultural University,Baoding 071000,China;Garden Management Centre of Hebei Province,Chengde 067000,China)

机构地区：[1]河北农业大学华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,保定071000 [2]河北省承德市园林管理中心,承德067000

出　　处：《农业生物技术学报》2023年第8期1567-1577,共11页Journal of Agricultural Biotechnology

基　　金：国家玉米产业技术体系(CARS-02);河北省自然科学基金(C2019204246);中央引导地方科技发展资金(216Z6501G,216Z6502G);河北省省属高校基本科研业务费研究项目(KY2021044)。

摘　　要：脂氧合酶(lipoxygenase,LOX)是植物体内重要的氧化还原酶,广泛参与植物生长和发育,响应生物与非生物胁迫等过程。目前,玉米(Zea mays)LOX家族成员及其在茉莉酸(jasmonic acid,JA)信号通路中的作用均未明确。为明确玉米LOX家族成员及其功能,本研究利用生物信息学方法,对玉米LOX家族基因进行了鉴定,分析了其系统进化、保守基序、组织表达特异性以及生物与非生物胁迫下的表达规律;利用qRT-PCR技术,检测了玉米LOX家族基因在禾谷镰刀菌(Fusarium graminearum)侵染和茉莉酸甲酯(methyl jasmonate,MeJA)处理下的表达规律。结果发现,玉米基因组中包含13条LOX基因,可分为9-LOX、13-LOX Type Ⅰ和13-LOX Type Ⅱ3类,并且全部包含保守的脂氧合酶基序;玉米LOX家族基因在不同组织与生物和非生物胁迫下的表达水平呈现显著差异(P<0.05);禾谷镰刀菌侵染和MeJA处理后,13条玉米LOX家族基因的表达量均显著上调(P<0.05),表明玉米LOX家族基因在玉米生长发育以及响应不同生物与非生物胁迫过程可能具有重要功能。本研究为深入了解玉米LOX家族基因的功能及其作用机制提供参考依据。Lipoxygenase(LOX)is an important oxidoreductase in plants,which is widely involved in plant growth,development and response to biological and abiotic stresses.At present,the members of maize(Zea mays)LOX family and their roles in jasmonic acid(JA)signaling pathway are still not clear.In order to explore the function of LOX family genes in maize,bioinformatics methods were used to identify maize LOX family genes,and analyzed their phylogenetic evolution,conserved motifs,tissue expression specificity and expression patterns under biological and abiotic stresses.The expression of maize LOX family genes inoculated with Fusarium graminearum and induced by methyl jasmonate(MeJA)were detected by qRT-PCR.The results showed that maize genome contained 13 LOX family genes,which could be divided into 9-LOX,13-LOX Type Ⅰ and 13-LOX Type Ⅱ,and all of them contained conservative lipoxygenase motif.The expression levels of ZmLOXs showed significant differences in different tissues or under different biological and abiotic stresses(P<0.05).After F.graminearum inoculation and MeJA treatment,the expression levels of 13 LOX family genes in maize were significantly up-regulated(P<0.05).These results suggested that maize LOX family genes might have important functions in maize growth and development and response to different biological and abiotic stresses.This study provides a reference for further understanding the function and mechanism of LOX family genes in maize.

关 键 词：脂氧合酶(LOX) 茉莉酸甲酯(MeJA) 生物信息学 玉米 

分 类 号：S432.1[农业科学—植物病理学]