基于水杨酸和茉莉酸拮抗作用的烟碱合成调节研究  

Study on the Regulation of Nicotine Synthesis Based on the Antagonism of Jasmonic Acid and Salicylic Acid

作　　者：李寒 蔡凯 高维常 李想 张恒 朱经伟 刘艳霞 LI Han;CAI Kai;GAOWei-Chang;LI Xiang;ZHANG Heng;ZHU Jing-Wei;LIU Yan-Xia(Guizhou Academy of Tobacco Science,Guiyang 550081,China;Guizhou Branch Company of China Tobacco Corporation,Guiyang 550004,China)

机构地区：[1]贵州省烟草科学研究院,贵阳550081 [2]中国烟草总公司贵州省公司,贵阳550004

出　　处：《农业生物技术学报》2023年第8期1595-1609,共15页Journal of Agricultural Biotechnology

基　　金：贵州省微生物与健康院士工作站(黔科合平台人才[2020]4004);中国烟草总公司贵州省公司科技项目(201802,201905,201906,2021XM17);贵州省烟草科学研究院科技项目(GZYK2021-11)。

摘　　要：在烟叶生产中,我国烟叶的烟碱含量不同程度偏高,致使烟叶的化学成分不协调,影响了烟叶的工业可用性,造成烟叶大量库存积压。因此,有效降低烤烟烟叶中的烟碱含量已成为了烟叶生产的重要议题。本研究基于打顶等机械损伤可通过激活茉莉酸(jasmonic acid,JA)信号途径以刺激烟株烟碱的合成为切入点,探究利用水杨酸(salicylic acid,SA)和JA在信号传导中的拮抗作用调节烟株烟碱的合成,揭示SA拮抗JA信号调节烟碱合成的可能途径。以‘云烟87’(Nicotiana tabacum cv.‘Yunyan 87’)为实验材料,待烟草生长至盛花期时,对其进行打顶、采叶等机械损伤处理,分析机械损伤处理后根施SA对烟碱合成相关基因的表达、烟株总氮和烟碱含量的影响;并对经JA、SA、JA+SA处理的烟苗根系进行转录组学分析。结果表明,多重机械损伤能够进一步诱导烟碱合成相关基因的表达,促进体内氮素向烟碱合成途径的转化;SA能拮抗JA信号,抑制烟碱合成相关基因的表达,阻碍体内氮素向烟碱合成途径的转化,降低烟株的烟碱含量;转录组学的共表达网络分析中,14个分别隶属于bHLH、AP2-EREBP和WRKY家族的转录因子不仅能被SA显著诱导,而且其表达模式与烟碱合成相关基因呈负相关关系;启动子元件分析显示,烟碱合成相关基因的启动子区域广泛分布着能被bHLH、AP2/ERF和WRKY识别的顺式作用元件,为bHLH、AP2-EREBP和WRKY转录因子参与SA拮抗JA信号途径,并通过靶向结合启动子区域以抑制烟碱合成相关基因的转录提供了可能。本研究为有效调节烟碱合成、改良烟叶质量提供了参考。In tobacco production,the nicotine content of tobacco leaves in China is high to varying degrees,resulting in inconsistent chemical composition of tobacco leaves,which affects the industrial availability of tobacco leaves and causes a large backlog of tobacco leaves in stock.Therefore,effective reduction of nicotine content in flue-cured tobacco leaves has become an important issue in tobacco production.Based on the fact that mechanical injury such as topping can stimulate nicotine synthesis in tobacco plants by activating the jasmonic acid(JA)signal,this study explored the possible pathway of salicylic acid(SA)antagonizing JA signal to regulate nicotine synthesis in tobacco plants by using the antagonistic effect of SA and JA in signal transduction.Using Nicotiana tabacum cv.‘Yunyan 87’as the experimental material,the tobacco plants were subjected to mechanical damage treatments,such as topping and leaf picking,when they were growing to full flowering stage.The effects of SA on the expression of nicotine biosynthesis-related genes and the content of total nitrogen and nicotine in the tobacco plants were analyzed,and the transcriptomic analysis of the tobacco roots treated with JA,SA and JA+SA was performed.The results showed that multiple mechanical injuries further induced the expression of nicotine biosynthesis genes and the conversion of nitrogen to nicotine biosynthesis pathway.In contrast,SA could antagonize JA signaling,inhibit the expression of nicotine biosynthesis genes,hinder the conversion of nitrogen to nicotine biosynthesis pathway,and reduce the nicotine content in tobacco plants.The transcriptomics-based co-expression network analysis showed that 14 transcription factors belonging to the bHLH,AP2-EREBP and WRKY families,respectively,were not only significantly induced by SA,but also their expression patterns were negatively correlated with nicotine biosynthesis genes.In addition,promoter cis-element analysis revealed that the promoters of nicotine biosynthesis-related genes were widely distrib

关 键 词：烟草 烟碱 茉莉酸 水杨酸 拮抗 

分 类 号：S184[农业科学—农业基础科学]