水稻氮高效基因OsTCP19分子标记的开发利用  

Development and Application of Molecular Marker for High Nitrogen Use Efficiency Gene OsTCP19 in Rice(Oryza sativa)

作　　者：郭发平 田敏 白大嵩 税阳 徐娜娜 余德金 彭友林[1] 胡运高[1] GUO Fa-Ping;TIAN Min;BAI Da-Song;SHUI Yang;XU Na-Na;YU De-Jin;PENG You-Lin;HU Yun-Gao(Rice Research Institute,Southwest University of Science and Technology,Mianyang 621010,China)

机构地区：[1]西南科技大学水稻研究所,绵阳621010

出　　处：《农业生物技术学报》2023年第8期1747-1756,共10页Journal of Agricultural Biotechnology

基　　金：四川省重大研发计划(2021YFYZ0016);四川省重大科技专项(2022ZDZX0012);四川省自然科学基金(2022NSFSC0153);国家自然科学基金(32001491)。

摘　　要：氮素是影响水稻(Oryza sativa)产量和品质最重要的元素。在水稻氮高效育种过程中,针对目的基因进行分子标记辅助选择有助于高效、便捷地筛选氮高效水稻品种。转录因子TCP(TEOSINTE BRANCHED1,CYCLOIDEA,PROLIFERATING CELL FACTORS)参与多种发育过程的调控。OsTCP19是新近报道的氮高效基因,其启动子区存在29 bp的缺失。本研究设计Pro TCP19-F/R引物进行分子标记开发,从而对水稻材料进行鉴定。对33个参考基因组材料的OsTCP19基因启动子区域进行比对分析,发现‘Tumba’、‘CG14’、‘IR64’、‘N22’、‘蜀恢548’、‘Basmati’和‘ZH11’共7个品种中存在OsTCP19启动子区域的29 bp缺失。针对该缺失序列设计引物,以‘蜀恢548’为阳性对照,对23个水稻育种亲本材料、测配F1代及其父本以及‘西科恢646’混合株系的OsTCP19基因型进行鉴定,结果显示,亲本材料‘西科恢1288’、‘Kasalath’、‘IRAT109’与‘蜀恢548’条带一致,缺失29 bp,鉴定为ostcp19突变基因型;‘西科恢1288’和‘西科恢646’的测配组合F1代含有双条带,鉴定为杂合型;‘西科恢646’单株鉴定能鉴定出2种基因型。因此,该标记鉴定方法可高效、准确地对OsTCP19基因型及种子纯度进行鉴定,鉴定方法简单、快捷、成本低廉,在水稻育种中具有潜在的推广价值。Nitrogen is the most important element affecting the yield and quality of rice(Oryza sativa).In the process of rice N-efficient breeding,molecular marker-assisted selection for target genes is helpful for efficient and convenient screening of N-efficient rice varieties.Transcription factor TCP(TEOSINTE BRANCHED1,CYCLOIDEA,PROLIFERATING CELL FACTORS)is involved in the regulation of various developmental processes.OsTCP19 is a newly reported nitrogen-efficient gene with 29 bp deletion in its promoter region.In this study,Pro TCP19-F/R was designed to develop molecular markers to identify rice materials.By comparing and analyzing the OsTCP19 promoter region of 33 reference genomic materials,it was found that 29 bp deletion of OsTCP19 promoter region was found in‘Tumba’,‘CG14’,‘IR64’,‘N22’,‘Shuhui 548’,‘Basmati’and‘ZH11’.Using‘Shuhui 548’as positive control,OsTCP19 genotypes of 23 rice breeding parents,F1 generation and its paternity,and‘Xikeshui 646’hybrid lines were identified.The results showed that‘Xikeshui1288’,‘Kasalath’,‘IRAT109’had a deletion of 29 bp,which was consistent with‘Shuhui548’and identified as ostcp19 mutant genotype.The test-cross F1 generation of‘Xikehui1288’and‘Xikehui646’contained 2 strips,which were identified as heterozygous type.Two genotypes could be identified by single plant identification of'Xikehui646'.Therefore,the marker identification method could identify OsTCP19 genotype and seed purity efficiently and accurately,and the identification method is simple,fast and low cost,which has potential popularization value in rice breeding.

关 键 词：水稻 TCP19(TEOSINTE BRANCHED1 CYCLOIDEA PROLIFERATING CELL FACTOR 19) 分子标记 氮高效 分子标记辅助育种 

分 类 号：S336[农业科学—作物遗传育种] S338[农业科学—农艺学]