Unravelling how biochar and dung amendments determine the functional structure and community assembly related to methane metabolisms in grassland soils  

作　　者：Qingzhou Zhao Yanfen Wang Zhihong Xu Juanli Yun Zhisheng Yu 

机构地区：[1]College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 100049,People’s Republic of China [2]College of Urban and Environmental Science,Peking University,Beijing 100871,People’s Republic of China [3]School of Environment and Science,Griffith University,Nathan,QLD 4111,Australia [4]Center of Planetary Health and Food Security,Griffith Univer-sity,Nathan,QLD 4111,Australia [5]Yanshan Earth Critical Zone and Surface Flux Observation Research Station,Chinese Academy of Sciences,Beijing 100049,People’s Republic of China [6]State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,People’s Republic of China [7]RCEES-IMCAS-UCAS Joint-Lab of Microbial Technology for Environmental Science,Beijing 100085,People’s Republic of China

出　　处：《Biochar》2022年第1期377-390,共14页生物炭（英文）

基　　金：the National Key Research and Development Program of China(No.2018YFD0800403).

摘　　要：Biochar and dung amendments have been extensively employed in soil remediation and fertilization of grasslands,which are the largest terrestrial sinks for methane.However,how these exogenous amendments regulate methane metabolisms at the molecular and community levels remains elusive.In this study,we investigated the functional genes and community assemblies of methanogens and methanotrophs using Geochip 5.0 and high-throughput sequencing to reveal the impacts of biochar and dung on soil methanogenesis and methane oxidation.The interac-tions between methane metabolic genes and other biogeochemical genes were also examined.According to Geo-chip microarrays,methanogenic gene mcrA decreased and increased with dung or biochar amendment,respectively;The methanotrophic gene pmoA showed a reverse but not significant tendency.Undominated processes contributed 65.51%to replace homogeneous selections as primary driving forces of methanogen assembly after dung amend-ment;the contribution of dispersal limitation increased to 46.13%in methanotroph assembly after biochar amend-ment.The diversity and association of co-occurrence networks for carbon-nitrogen cycling genes decreased after exogenous amendments.These results indicated that biochar and dung amendments prominently regulated the functional genes and community assembly involved in methane metabolisms.The co-existence patterns of methane metabolic genes and other related geochemical genes were also shaped by these amendments.This study provides the scientific reference for the development of grassland management in the context of global warming.

关 键 词：Biochar amendment Dung amendment Methane metabolism Nitrogen cycling Community assembly Geochip 5.0 

分 类 号：S15[农业科学—土壤学]