Two NIS1-like proteins from apple canker pathogen(Valsa mali)play distinct roles in plant recognition and pathogen virulence  

作　　者：Jiajun Nie Wenjing Zhou Yonghui Lin Zhaoyang Liu Zhiyuan Yin Lili Huang 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Plant Protection,Northwest A&F University,3 Taicheng Road,Yangling 712100,Shaanxi,China [2]College of Plant Protection,China Agricultural University,Beijing 100193,China

出　　处：《Stress Biology》2022年第1期606-620,共15页逆境生物学（英文）

基　　金：supported by China Postdoctoral Science Foundation(2021 M690128);the Open Project Program of State Key Laboratory of Crop Stress Biology for Arid Areas(CSBAA2020011);National Natural Science Foundation-Xinjiang Joint Foundation of China(U1903206);Major Scientific and Technological Projects of Shaanxi Province(2020zdzx03-03-01).

摘　　要：Conserved effectors produced by phytopathogens play critical roles in plant-microbe interactions.NIS1-like proteins represent a newly identified family of effectors distributed in multiple fungal species.However,their biological functions in a majority of pathogenic fungi remain largely elusive and require further investigation.In this study,we characterized two NIS1-like proteins VmNIS1 and VmNIS2 from Valsa mali,the causal agent of apple Valsa canker.Both of these two proteins were predicted to be secreted.Using agroinfiltration,we found that VmNIS1 induced intense cell death,whereas VmNIS2 suppressed INF1 elicitin-triggered cell death in Nicotiana benthamiana.Treatment of N.benthamiana with VmNIS1 recombinant protein produced by Escherichia coli activated a series of immune responses and enhanced plant disease resistance against Phytophthora capsici.In contrast,VmNIS2 suppressed plant immune responses and promoted P.capsici infection when transiently expressed in N.benthamiana.Both VmNIS1 and VmNIS2 were shown to be highly induced at late stage of V.mali infection.By individually knocking out of these two genes in V.mali,however,only VmNIS2 was shown to be required for pathogen virulence as well as tolerance to oxidative stress.Notably,we further showed that C-terminal extension of VmNIS1 was essential for plant recognition and VmNIS2 may escape plant detection via sequence truncation.Our data collectively indicate that VmNIS1 and VmNIS2 play distinct roles in plant recognition and pathogen virulence,which provided new insights into the function of NIS1-like proteins in plant-microbe interactions.

关 键 词：Valsa mali Effector protein Cell death Plant immunity VIRULENCE 

分 类 号：S436.611[农业科学—农业昆虫与害虫防治]