藤龙补中颗粒对CT26大肠癌转移的影响及机制  

Effect and Mechanism of Tenglong Buzhong Granules on Metastasis of CT26 ColorectalCancer

作　　者：黄晓伟[1] 陈锦芳 邬世威 师子曼 胡兵[1] HUANG Xiaowei;CHEN Jinfang;WU Shiwei;SHI Ziman;HU Bing

机构地区：[1]上海中医药大学附属龙华医院(上海中医药大学中医肿瘤研究所)肿瘤科,上海200032

出　　处：《新中医》2023年第11期156-159,共4页New Chinese Medicine

基　　金：上海市科学技术委员会科研计划项目(16401902500);上海市自然科学基金项目(20ZR1458700)。

摘　　要：目的:观察藤龙补中颗粒对CT26大肠癌转移的作用。方法:BALB/c小鼠尾静脉注射CT26细胞,随机分为对照组、藤龙补中颗粒小剂量和大剂量组,给予无菌水或小剂量和大剂量藤龙补中颗粒灌胃,每天1次,治疗21 d。治疗结束后,取肺称质量、计数肺表面转移结节。苏木精-伊红染色法(HE)染色观察转移灶。Western blot检测蛋白表达。结果:与对照组比较,藤龙补中颗粒治疗后,大小剂量组肺表面转移结节减少、肺质量降低(P<0.01)。与对照组比较,大小剂量组基质金属蛋白酶-2 (MMP-2)、MMP-9表达均下调(P<0.01)。与对照组比较,大小剂量组Wnt蛋白3 (Wnt3)、CTNNB1蛋白表达下调(P<0.01)。结论:藤龙补中颗粒可以抑制CT26大肠癌转移,其作用机制可能与抑制Wnt/β-catenin信号转导、降低MMP-2和MMP-9表达有关。Objective:To observe the effect of Tenglong Buzhong Granules on the metastasis of CT26 colorectal cancer.Methods:CT26 cells were injected into the tail vein of BALB/c mice.They were randomly divided into control group,small dose and large dose groups of Tenglong Buzhong Granules.They were given sterile water or small dose and large dose of Tenglong Buzhong Granules by gavage once a day for 21 days.After treatment,taking the lung to weigh the mass and count the metastatic nodules on the lung surface.Hematoxylin-eosin staining(HE)was used to observe the metastasis.Western blot was used to detect the protein expression.Results:Compared with the control group,after the treatment with Tenglong Buzhong Granules,the number of metastatic nodules on the lung surface and the quality of lung in the large and small dose groups were decreased(P<0.01).Compared with the control group,the expressions of matrix metalloproteinase-2(MMP-2)and MMP-9 in the large and small dose groups were down-regulated(P<0.01).Compared with the control group,the expressions of Wnt protein 3(Wnt3)and CTNNB1 protein in the large and small dose groups were down-regulated(P<0.01).Conclusion:Tenglong Buzhong Granules can inhibit the metastasis of CT26 colorectal cancer,and its mechanism may be related to the inhibition of Wnt/β-catenin signal transduction and decreased expressions of MMP-2 and MMP-9.

关 键 词：大肠癌 转移 藤龙补中颗粒 Wnt3 Β-CATENIN 基质金属蛋白酶 

分 类 号：R285.5[医药卫生—中药学]