甘氨酸溶液应用于冰冻解冻去甘油红细胞制备的研究  

Study of glycine solution in preparation of frozen thawed deglycerolized erythrocytes

作　　者：王道琳 卓海龙 刘慧 陈婷婷 骆群 WANG Daolin;ZHUO Hailong;LIU Hui;CHEN Tingting;LUO Qun(Department of Transfusion Medicine,Fifth Medical Center of Chinese PLA General Hospital,Beijing 100071,China)

机构地区：[1]中国人民解放军总医院第五医学中心输血医学科,北京100071

出　　处：《中国输血杂志》2023年第7期583-586,共4页Chinese Journal of Blood Transfusion

摘　　要：目的探讨甘氨酸溶液对冰冻红细胞解冻过程的保护作用。方法选择采集日期在6 d内的去白细胞悬浮红细胞1 U共20袋进行研究。每2袋悬浮红细胞混匀后均分成2袋,分为两组;每组10袋,每袋均为1 U,进行冰冻保存。其中一组使用氯化钠溶液进行去甘油处理(对照组),一组使用甘氨酸溶液进行去甘油处理(实验组)。检测两组解冻去甘油红细胞的血红蛋白、游离血红蛋白、甘油残留量、红细胞内甘油总量、三磷酸腺苷(ATP)和2,3-二磷酸甘油酸(2,3-DPG)。结果与对照组游离血红蛋白含量(0.90±0.05)g/L、甘油残留量(1.17±0.08)g/L相比,实验组终产品红细胞上清游离血红蛋白含量(0.77±0.15g/L)、甘油残留量(0.79±0.33)g/L含量下降,差异有统计学意义(P<0.05);与对照组ATP含量(4.03±0.38)μmol/gHb和2,3-DPG含量(485.65±78.08)μg/L相比,实验组ATP含量(4.41±0.35)μmol/gHb和2,3-DPG含量(656.28±116.68)μg/L明显升高,差异有统计学意义(P<0.05)。结论使用甘氨酸溶液替代氯化钠溶液制备冰冻解冻去甘油红细胞,达到了保护红细胞的效果,降低了红细胞溶血率和甘油残留量,提升了红细胞的回收率。Objective To study the protective effect of glycine solution on frozen red blood cell thawing process.Methods A total of 20 bags of 1 U of leukocytes reduced suspended red blood cells within 6 days were selected for the study.After mixing,each 2 bags of suspended red blood cells were divided into 2 bags and into two groups with 10 bags of 1 U in each group,and were frozen for storage.One group was deglycerolized with sodium chloride solution(control group),and one group was deglycerolized with glycine solution(experimental group).The hemoglobin,free hemoglobin,residual glycerol,total glycerol in red blood cells,adenosine triphosphate(ATP)and 2,3-diphosphoglycerate(2,3-DPG)were detected in the two groups.Results Compared with the free hemoglobin content(0.90±0.05)g/L and residual glycerol content(1.17±0.08)g/L in the control group,the final product red blood cell supernatant free hemoglobin content(0.77±0.15)g/L and residual glycerol content(0.79±0.33)g/L in the experimental group were decreased,and the difference was statistically significant(P0.05).Compared with the ATP content(4.03±0.38)μmol/gHb and 2,3-DPG content(485.65±78.08)μg/L in the control group,the ATP content(4.41±0.35)μmol/gHb and 2,3-DPG content(656.28±116.68)μg/L in the experimental group were significantly increased,with statistical significance(P0.05).Conclusion Using glycine solution instead of sodium chloride solution to prepare frozen thawed deglycerolized erythrocytes achieved the effect of protecting erythrocytes,reduced the hemolysis rate of erythrocytes and glycerin residue,and increased the recovery rate of erythrocytes.

关 键 词：甘氨酸 红细胞 ATP 2 3-DPG 溶血率 回收率 

分 类 号：R457.12[医药卫生—治疗学] R331.141[医药卫生—临床医学]