陕西地区血小板供者基因数据库拓展性应用的研究  

Study on the extensibility of platelet donor gene database in Shaanxi

作　　者：齐珺[1] 曹晓莉[1] 胡欣 李凤琴[1] 孙振东[1] 李昱辉 王满妮[1] 王天菊[1] 武君华[1] 尚利侠 陈乐 徐华[1] QI Jun;CAO Xiaoli;HU Xin;LI Fengqin;SUN Zhendong;LI Yuhui;WANG Manni;WANG Tianju;WU Junhua;SHANG Lixia;CHEN Le;XU Hua(Blood Center of Shaanxi Province,Institute of Xi′an Blood Bank,Xi'an 710061,China)

机构地区：[1]陕西省血液中心西安市中心血站,陕西西安710061

出　　处：《中国输血杂志》2023年第7期637-641,共5页Chinese Journal of Blood Transfusion

基　　金：陕西省重点研发计划一般项目(2022SF-098);西安市卫生健康委员会一般研究项目(2022yb06)。

摘　　要：目的通过分析本地区无偿献血与造血干细胞捐献群体之间的共性和特性,挖掘包括血小板基因供者库在内的无偿献血者与造血干细胞捐献者资料库间的融合潜力,提高招募成功率和已知结果库存率。方法应用数据库建模和比对方法对本市近10年无偿献血者与中华骨髓库陕西分库造血干细胞捐献者之间的匹配度和融合度进行筛选分层,采用Arlequin 3.5.2.2软件计算HLA、HPA基因频率及单体型频率,并根据表型频率计算不同血小板供者后备库中找到HLA、HPA全匹配供者概率。结果在本地区已入库造血干细胞志愿捐献者中,依据其献血行为挖掘出的活跃献血者分别划分为血小板供者库后备一、二、三、四梯队,分别为696名、2752名、9092名和12028名捐献者。第一梯队具有10~50次血小板捐献经历和10~20次全血捐献经历的占比最高,分别为13.65%和26.01%;第二梯队具有10~20次全血捐献经历和10~50次血小板捐献经历者占比分别为15.04%和1.38%,与其他梯队献血特征有差异(P<0.05)。在现有血小板库+一二梯队的核心库容中(n=4955),患者找到至少1名HLA-A,B表型相同的供者的概率可达69.02%;当考虑到ABO和HPA同型时,找到至少1名HLA、HPA全相合且ABO同型供者概率(B型为例)为48.73%。结论西安地区全血捐献、单采血小板捐献和造血干细胞捐献这3类群体存在较大交叉分布性,与从头开始扩充库容相比,造血干细胞捐献者资料库中的活跃献血者是血小板供者库的后备有声力量,在扩大有效库容的同时能够最大限度降低建库成本和资源需求。Objective To analyze the commonality and characteristics between voluntary blood donors and hematopoietic stem cell donors in this region,and explore the potential for integration and development between China Marrow Donors Program(CMDP)and voluntary blood donors,especially platelet donor databases,so as to improve recruitment success rate and inventory rate.Methods The database modeling and comparison methods were used to screen and stratify the matching and integration degree between the voluntary blood donors in recent 10 years and the marrow donors in the Shaanxi Branch of CMDP.The frequencies of HLA-A,-B alleles,HPA alleles and haplotypes were calculated with Arlequin 3.5.2.2 software,and the matching probability of different platelet donor reserve pools was conducted according to the phenotypic frequencies.Results Among the voluntary donors with known HLA genotypes in this region,according to their blood donation behavior,the active blood donors excavated were divided into the first,second,third and fourth echelons of platelet donor reserve pools,with 696,2752,9092 and 12028 donors,respectively.The first echelon had the highest proportion of 10-50 times of platelet donations and 10-20 times of whole blood donations,with 13.65%and 26.01%,respectively.The second echelon had 10-20 times of whole blood donations and 10-50 times of platelet donations,accounted for 15.04%and 1.38%,respectively,which were significantly different from other echelons′blood donation characteristics(P0.05).With a database size of the existing platelet donor bank adding the first and second echelons(n=4955),there was a 69.02%probability of matching at least one donor with matching HLA-A-B phenotype.When considering the matching ABO and HPA phenotypes,the probability of finding at least one donor with fully matching HLA,HPA and ABO isotype(type B as an example)was 48.73%.Conclusion The three groups of whole blood donation,apheresis platelet donation and marrow donation in Xi′an area have a large cross-distribution.Compared with expa

关 键 词：人类白细胞抗原 血小板特异性抗原 血小板输注无效 造血干细胞捐献者资料库 血小板基因供者库 

分 类 号：R457.11[医药卫生—治疗学] R331.143[医药卫生—临床医学]