蜂蜜对大鼠血尿酸水平及肾损伤研究  

Effects of scacia honey on serum uric acid level and renal injury in rats

作　　者：许秀荷 何晓丽 周佳顺 潘丽珠 周卓君 李佳悦 王彩霞 袁伟 朱桂琦 XU Xiuhe;HE Xiaoli;ZHOU Jiashun;PAN Lizhu;ZHOU Zhuojun;LI Jiayue;WANG Caixia;YUANWei;ZHU Guiqi(Department of Pediatrics,Shanghai Jing'an Shibei Hospital,Shanghai 200443,China;Department of Nutrition,Shanghai Jing'an District Pengpu Town Second Community Health Service Cente,Shanghai 200436,China;Department of Nutrition,Shanghai Jing'an District Zhabei Central Hospital,Shanghai 200070,China)

机构地区：[1]上海市静安区市北医院,上海200443 [2]上海市静安区彭浦镇第二社区卫生服务中心,上海200436 [3]上海市静安区闸北中心医院,上海200070

出　　处：《中国临床药理学与治疗学》2023年第7期743-750,共8页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：上海市静安区重要薄弱学科建设(2021BR06)。

摘　　要：目的:观察氧嗪酸钾模型大鼠饮用洋槐蜂蜜(AH)水溶液后,对大鼠血尿酸水平及肾功能的影响。方法:雄性SD大鼠60只,随机分为空白对照组(CON组)、氧嗪酸钾模型组(OA模型组)、10%果糖组(10%F组)以及不同浓度蜂蜜组(25%、12.5%及6.25%AH组)。所有大鼠正常饲料喂养,CON对照组每日皮下注射5%羧甲基纤维素钠(CMC-Na)溶液同时饮用无菌水,其余各组大鼠每日皮下注射100 mg/kg OA CMC-Na混悬溶液,同时饮用无菌水、果糖或不同浓度AH水溶液。本实验为期4周。实验前及实验期间大鼠每周称重并取血一次,实验结束后取血、尿、粪便及肾脏组织,测定血、尿、粪便中尿酸含量、血清肌酐(Cre)及尿素氮(BUN)水平及肾脏组织炎症因子,并进行肾脏组织病理切片观察。结果:与空白对照组相比,蜂蜜可显著降低大鼠体质量(P<0.05),增加大鼠肾脏脏器系数,升高大鼠血尿酸、尿尿酸(UUA)、血清肌酐及尿素氮水平,降低大鼠粪尿酸(FUA)水平;蜂蜜可下调大鼠肾脏组织肿瘤坏死因子-α(TNF-α)水平(P<0.05)、上调单核细胞趋化蛋白1(MCP-1)及转化生长因子β1(TGF-β1)表达水平;蜂蜜能引起大鼠肾脏组织肾小管轻微到轻度扩张及轻到中度嗜碱性病变,并具有剂量依赖性。结论:在研究剂量范围内,蜂蜜能引起大鼠高尿酸血症、肾小管扩张及嗜碱性病变,导致肾功能损伤。AIM:To observe the effect ofacacia honey(AH)on serum uric acid level and renal function in potassium oxonate modelrats after drinking AH aqueous solution.METHODS:Sixty male SD rats were selected and randomly divided into control group(CON group),potassium oxonate model group(OA model group),10%fructose group(10%F group)and different concentration honey groups(25%,12.5%and 6.25%AH groups).All rats were fed with normal diet.The rats in CON group were subcutaneously injected with 5%sodium carboxymethyl cellulose(CMC-Na)solution and drunk sterile water every day,while rats in other groups were injected with 100 mg/kg OA solution suspended with 5%CMC-Na subcutaneouslyand drunksterile water orfructose solution or AH solution of different concentrations every day.Before and during the 4-week test,rats were weighed and blood was taken once a week.At the end of test,urine and feces specimens or kidney tissues were collected and blood was taken from the abdominal aorta.The uric acid content in blood,urine,and feces and the levels of serum creatinine(Cre)and blood urea nitrogen(BUN)or inflammatory factors in kidney tissues were measured.Renal function and histology were evaluated.RESULTS:Compared with CON group,AH could significantly reduce the body weight of rats(P<0.05),increase the kidney organ coefficient,the levels of serum uric acid,and uric acid in urine or feces,and reduce the level of fecal uric acid(FUA)in rats.AH can down regulate the level of tumor necrosis factor alpha(TNF-a)(P<0.05)and up regulate the expression of monocyte chemoattractant protein 1(MCP-1)and transforming growth factorβ-1(TGF-β1)in rats kidneys;AH can cause slight to mild dilatation of renal tubules and mild to moderate basophilic lesions of renal rubules in rat kidney in a dose dependent manner.CONCLUSION:In the doses rang of present study,AH can cause hyperuricemia,renal tubular dilatation and basophilic lesions,and lead to renal function damage in rats.

关 键 词：洋槐蜂蜜 氧嗪酸钾 果糖 尿酸 大鼠 

分 类 号：R586[医药卫生—内分泌]