肝脏X受体(LXRα)对奶牛子宫内膜炎调节机制的研究  

Study of the regulatory mechanism of liver X receptor(LXRα)on endometritis in dairy cows

作　　者：赵立香[1] 李荣权[1] ZHAO Lixiang;LI Rongquan(Jilin Agricultural Science and Technology University,Jilin 132101,China)

机构地区：[1]吉林农业科技学院,吉林吉林132101

出　　处：《黑龙江畜牧兽医》2023年第13期68-73,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：吉林省教育厅科学技术研究规划项目(JJkH20210411kJ)。

摘　　要：为了探索肝脏X受体(LXRα)对脂多糖(LPS)诱导的奶牛子宫内膜上皮细胞炎症反应的调节机制,研究采用小鼠体内试验及奶牛子宫内膜上皮细胞体外试验,用LXRα激动剂T0901317激活LXRα,子宫灌注0.2 mg/mL LPS 50μL,检测奶牛子宫内膜上皮细胞活性、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、亚硝酸盐、前列腺素E_(2)(PGE_(2))等炎性细胞因子的表达情况,对核因子κB(NF-κB)、糖原合成酶激酶3β(GSK3β)-重组与合成蛋白(Nrf2)信号通路的影响。结果表明:10,20,40μmol/L的T0901317对奶牛子宫内膜上皮细胞没有毒性作用,对TNF-α、IL-1β、亚硝酸盐、PGE_(2)的表达具有极显著的抑制作用(P<0.01),可降低磷酸化蛋白65(p-p65)、磷酸化蛋白ⅠκB(p-ⅠκB)的磷酰化水平,提高磷酸化葡萄糖合成激酶3β(p-GSK3β)、Nrf2及Nrf2调控的抗氧化因子血红素氧化酶-1(HO-1)的表达。说明LXRα可以通过调节GSK3β-Nrf2信号通路抑制LPS诱导的子宫内膜炎症。In order to explore the regulatory mechanism of liver X receptor(LXRα)on lipopolysaccharide(LPS)induced inflammatory response in dairy cow endometrial epithelial cells,in this experiment,in vivo test of mice and in vitro test of dairy cow endometrial epithelial cells were employed;LXRαwas activated with LXRαagonist T0901317 and uterine perfusion of 0.2 mg/mL LPS 50μL was carried out for the determination of dairy cow endometrial epithelial cell activity,the expression of inflammatory cytokines such as tumor necrosis factorα(TNF-α),interleukin-1β(IL-1β),nitrite,and prostaglandin E_2(PGE_2),as well as its effect on nuclear factorκB(NF-κB),glycogen synthetase kinase 3β(GSK3β)-synthetic protein(Nrf2)signaling pathways.The results showed that T0901317 of 10,20,40μmol/L had no toxic effect on dairy cow endometrial epithelial cells,and had very significant inhibitory effect on the expression of TNF-α,IL-1β,nitrite and PGE_2(P<0.01),which could reduce the phosphorylation levels of phosphorylated protein 65(p-p65)and phosphorylated proteinⅠκB(p-ⅠκB),and increase the expression of phosphorylated glucose synthesis kinase 3β(p-GSK3β),recombinant and Nrf2 and Nrf2-regulated antioxidant factor heme oxidase-1(HO-1).The results suggested that LXRαcould inhibit LPS-induced endometrial inflammation by regulating the GSK3β-Nrf2 signaling pathway.

关 键 词：肝脏X受体(LXRα) 脂多糖 奶牛子宫内膜上皮细胞 LXRα激动剂T0901317 炎症反应 调节机制 

分 类 号：S858.23[农业科学—临床兽医学]