肝素亲和层析介质制备方法及应用研究进展  

Research Progress on Preparation Methods of Heparin Affinity Chromatography Medium

作　　者：古嘉如 穆士凯 武玉哲 靳海波[1] 何广湘[1] 张荣月[1] GU Jiaru;MU Shikai;WU Yuzhe;JIN Haibo;HE Guangxiang;ZHANG Rongyue(College of New Materials and Chemical Engineering,Beijing Institute of Petrochemical Technology,Beijing Key Laboratory of Fuel Cleanliness and Efficient Catalytic Emission Reduction Technology,Beijing 102617,China)

机构地区：[1]北京石油化工学院新材料与化工学院,燃料清洁化及高效催化减排技术北京市重点实验室,北京102617

出　　处：《离子交换与吸附》2023年第2期172-188,共17页Ion Exchange and Adsorption

基　　金：北京市委组织部青年拔尖人才项目(ZZB2019001)。

摘　　要：肝素亲和层析是利用生物大分子与层析介质表面的肝素特异性结合而进行选择性分离的一种技术,使用条件温和,纯化过程中能较好保持目标蛋白的生物活性。本文在阐述肝素与层析介质化学反应机理的基础上,系统论述了溴化氰活化及偶联法、环氧活化及偶联法、席夫碱法和碳二亚胺缩合法四种肝素亲和层析介质制备方法,深入分析了各制备方法的优缺点,总结了肝素亲和层析在血液制品、病毒纯化方面的应用。最后,进一步分析了肝素亲和层析在蛋白分离纯化领域应用存在的问题,并展望了其未来发展趋势,以期为肝素亲和层析介质的制备方法提供新思路。Heparin affinity chromatography is a selective separation technique that utilizes the specific binding of biological macromolecules to heparin on the surface of the medium.It can maintain the biological activity of the target protein in the process of purification under mild conditions.In this paper,based on the mechanism of chemical reaction between heparin and chromatography medium,and four kinds of the current preparation methods for heparin affinity chromatography were systematically discussed,including cyanogen bromide activation and coupling method,epoxy activation and coupling method,schiff base method and carbodimide condensation method.It was summarized that the heparin affinity chromatography was applied to blood purification products and virus.Meanwhile,the advantages and disadvantages of each preparation method was described.Finally,in order to provide new ideas for the preparation methods for heparin affinity chromatography medium,the present problems and future development of heparin affinity chromatography in the field of protein separation and purification were further prospected.

关 键 词：肝素亲和层析 制备方法 蛋白分离纯化 病毒分离纯化 

分 类 号：O658.1[理学—分析化学]