HILIC-HPLC法检测三乙烯四胺铜的含量  

Determination of triethylenetetramine copper by HILIC-HPLC

作　　者：廖欣怡 曹雅 杨雅楠 仇丽颖 LIAO Xing-yi;CAO Ya;YANG Ya-nan;QIU Li-ying(School of Pharmacy,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学药学院,四川成都610225

出　　处：《西南民族大学学报（自然科学版）》2023年第4期408-413,共6页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：国家自然科学基金项目(81300107)。

摘　　要：建立了一种测定三乙烯四胺铜含量的高效液相色谱法.对HPLC法的色谱条件进行了优化:以Agilent ZORBAX RR HILIC Plus(4.6 mm×100 mm, 3.5μm)为色谱柱,以乙腈-水为流动相进行梯度洗脱,流速为1 mL/min,检测波长为260 nm,柱温为室温.结果表明,三乙烯四胺铜浓度在0.05~1.0 mg/mL范围内与峰面积的线性关系良好,线性方程为y=6×106x-34901,相关系数r为0.999 2;平均加样回收率为99.0%,RSD为1.43%.此方法操作简单,结果准确,可作为三乙烯四胺铜的含量测定方法.A method for the determination of Triethylenetetramine copper was developed and validated.The chromatographic conditions of HPLC method were optimized:Chromatography was developed on an Agilent ZORBAX RR HILIC silica column(4.6 mm×100 mm,3.5μm)with acetonitrile and water as the mobile phase.The flow rate was 1 mL/min and the detection wavelength was 260 nm.The column temperature was room temperature.The methodological study showed that the calibration curve was linear in the range of 0.05~1.0 mg/mL(r=0.9992).The average recovery was 99.0%with RSD of 1.43%.This method is simple,accurate and suitable for quality of control of Triethylenetetramine copper.

关 键 词：高效液相色谱法 亲水作用色谱 三乙烯四胺铜 含量测定 

分 类 号：R927.1[医药卫生—药学]