SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus  

作　　者：ZHANG Hao-yang YANG Yan-fang GUO Feng SHEN Xiao-rui LU Shan CHEN Bao-shan 

机构地区：[1]College of Agriculture/Guangxi Key Laboratory of Sugarcane Biology,Guangxi University,Nanning 530004,P.R.China [2]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/College of Life Science and Technology,Guangxi University,Nanning 530004,P.R.China [3]Ministry and Province Co-sponsored Collaborative Innovation Center for Sugarcane and Sugar Industry,Guangxi University,Nanning 530004,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第7期2126-2137,共12页农业科学学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(31872635);the Guangxi Key Laboratory of Sugarcane Biology,China(2018-266-Z01);the Department of Science and Technology of Guangxi Zhuang Autonomous Region,China(AD17129002).

摘　　要：Sugarcane smut caused by Sporisorium scitamineum is a destructive disease responsible for significant losses in sugarcane production worldwide.However,the mechanisms underlying the pathogenicity of this fungus remain largely unknown.In this study,we found that the disruption of the SsRSS1 gene,which encodes a salicylic acid(SA)sensing regulator,does not affect phenotypic traits such as the morphology,growth rate,and sexual mating ability of haploid basidiospores,but rather reduces the tolerance of basidiospores to SA stress by blocking the induction of SsSRG1,a gene encoding a SA response protein in S.scitamineum.SsRSS1 deletion resulted in the attenuation of the virulence of the fungus.In addition to a significant reduction in whip formation,a portion of plantlets(18.3%)inoculated with theΔSsRSS1 strains were found to be infected but failed to produce whips for up to 90 days post-inoculation.However,the development of hyphae and teliospore from theΔSsRSS1-infected plants that formed whips seemed indistinguishable from that in the wild-type-infected plants.Combined,our findings suggested that SsRss1 is required for maintaining fungal fitness in planta by counteracting SA stress.

关 键 词：Sporisorium scitamineum SsRSS1 PATHOGENICITY salicylic acid response 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]