Mutagenesis of odorant coreceptor Orco reveals the distinct role of olfaction between sexes in Spodoptera frugiperda  

作　　者：CAO Song SUN Dong-dong LIU Yang YANG Qing WANG Gui-rong 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,P.R.China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第7期2162-2172,共11页农业科学学报（英文版）

基　　金：the National Natural Science Foundation of China(32130089);the China Postdoctoral Science Foundation(2020M680785);the Shenzhen Science and Technology Program,China(KQTD20180411143628272);the Central Public-interest Scientific Institution Basal Research Fund of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202108);the Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District,China(PT202101-02);the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIP).

摘　　要：Odorant receptor(OR)is crucial for insects to detect and recognize external chemical cues closely related to their survival.The insect OR forms a heteromeric complex composed of a ligand-specific receptor and a ubiquitously odorant receptor coreceptor(Orco).This study used the CRISPR/Cas9 technique to knock out(KO)Orco and reveal its essential role in acting on OR-meditated olfactory behavior in a critical invasive agricultural pest,the fall armyworm(FAW),Spodoptera frugiperda.Electroantennogram(EAG)results suggested that the Orco mutants of both male and female moths severely reduced their electrophysiological responses to the eight tested plant volatiles and two sex pheromones.However,the Orco gene played distinct roles in mating behavior between sexes:the mating behavior was fully disrupted in mutated males but not in mutated females.The oviposition result indicated that the Orco KO females displayed reduced egg laying by 24.1%compared with the mated wild type(WT)females.Overall,these results strongly suggest that Orco is an excellent target for disrupting FAW’s normal behavior and provides a feasible pest control approach.

关 键 词：Spodoptera frugiperda Orco CRISPR/Cas9 EAG olfactory behavior 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]