Dynamic chromatin architectures provide insights into the genetics of cattle myogenesis  

作　　者：Jie Cheng Xiukai Cao Xiaogang Wang Jian Wang Binglin Yue Wei Sun Yongzhen Huang Xianyong Lan Gang Ren Chuzhao Lei Hong Chen 

机构地区：[1]College of Animal Science and Technology,Northwest A&F University,No.22 Xinong Road,Yangling district,Yangling,Shaanxi province 712100,China [2]Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China,Yangzhou University,Yangzhou 225009,China [3]Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization,Southwest Minzu University,Chengdu 610225,China [4]College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China [5]College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China

出　　处：《Journal of Animal Science and Biotechnology》2023年第4期1344-1362,共19页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China[Grant No.31972558];the Agricultural Improved Seed Project of Shandong Province[Grant No.2020LZGC014-03]。

摘　　要：Background Sharply increased beef consumption is propelling the genetic improvement projects of beef cattle in China.Three-dimensional genome structure is confirmed to be an important layer of transcription regulation.Although genome-wide interaction data of several livestock species have already been produced,the genome structure states and its regulatory rules in cattle muscle are still limited.Results Here we present the first 3D genome data in Longissimus dorsi muscle of fetal and adult cattle(Bos taurus).We showed that compartments,topologically associating domains(TADs),and loop undergo re-organization and the structure dynamics were consistent with transcriptomic divergence during muscle development.Furthermore,we annotated cis-regulatory elements in cattle genome during myogenesis and demonstrated the enrichments of promoter and enhancer in selection sweeps.We further validated the regulatory function of one HMGA2 intronic enhancer near a strong sweep region on primary bovine myoblast proliferation.Conclusions Our data provide key insights of the regulatory function of high order chromatin structure and cattle myogenic biology,which will benefit the progress of genetic improvement of beef cattle.

关 键 词：CATTLE Cis-regulatory elements MUSCLE Selection sweep 3D genome 

分 类 号：S823.2[农业科学—畜牧学]