Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway  

作　　者：Ye Eun Joo Pil-Soo Jeong Sanghoon Lee Se-Been Jeon Min-Ah Gwon Min Ju Kim Hyo-Gu Kang Bong-Seok Song Sun-Uk Kim Seong-Keun Cho Bo-Woong Sim 

机构地区：[1]Futuristic Animal Resource and Research Center,Korea Research Institute of Bioscience and Biotechnology,Cheongju,South Korea [2]Department of Animal Science,College of Natural Resources and Life Science,Pusan National University,Miryang,South Korea [3]Laboratory of Theriogenology,College of Veterinary Medicine,Chungnam National University,Daejeon,South Korea [4]Department of Biotechnology,College of Engineering,Daegu University,Gyeongsan,South Korea [5]Department of Animal Science and Biotechnology,College of Agriculture and Life Science,Chungnam National University,Daejeon,South Korea [6]Department of Functional Genomics,University of Science and Technology,Daejeon,South Korea [7]Department of Animal Science,College of Natural Resources and Life Science,Life and Industry Convergence Research Institute,Pusan National University,Miryang,South Korea

出　　处：《Journal of Animal Science and Biotechnology》2023年第4期1395-1407,共13页畜牧与生物技术杂志（英文版）

基　　金：supported by the Ministry of Education;Science and Technology(No.2021M3A9A1096894);Republic of Korea and the KRIBB Research Initiative Program(KGM4252223);Korea Research Institute of Bioscience and Biotechnology,Republic of Korea。

摘　　要：Background Anethole(AN)is an organic antioxidant compound with a benzene ring and is expected to have a positive impact on early embryogenesis in mammals.However,no study has examined the effect of AN on porcine embryonic development.Therefore,we investigated the effect of AN on the development of porcine embryos and the underlying mechanism.Results We cultured porcine in vitro-fertilized embryos in medium with AN(0,0.3,0.5,and 1 mg/mL)for 6 d.AN at 0.5 mg/mL significantly increased the blastocyst formation rate,trophectoderm cell number,and cellular survival rate compared to the control.AN-supplemented embryos exhibited significantly lower reactive oxygen species levels and higher glutathione levels than the control.Moreover,AN significantly improved the quantity of mitochondria and mitochondrial membrane potential,and increased the lipid droplet,fatty acid,and ATP levels.Interestingly,the levels of proteins and genes related to the sonic hedgehog(SHH)signaling pathway were significantly increased by AN.Conclusions These results revealed that AN improved the developmental competence of porcine preimplantation embryos by activating SHH signaling against oxidative stress and could be used for large-scale production of high-quality porcine embryos.

关 键 词：ANETHOLE Lipid metabolism Mitochondrial function Porcine embryo development Sonic hedgehog signaling pathway 

分 类 号：S828[农业科学—畜牧学]