Yeast hydrolysate attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage in weaned piglets  

作　　者：Runqi Fu Chan Liang Daiwen Chen Gang Tian Ping Zheng Jun He Jie Yu Xiangbing Mao Yuheng Luo Junqiu Luo Bing Yu 

机构地区：[1]Key Laboratory of Animal Disease-Resistance Nutrition,Ministry of Education,Ministry of Agriculture and Rural Affairs,Key Laboratory of Sichuan Province,Chengdu 611130,China [2]Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

出　　处：《Journal of Animal Science and Biotechnology》2023年第4期1569-1583,共15页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Key Research and Development Program of China(2018YFD0500605);the Key Research and Development Program of Sichuan Province(2021YFYZ0008);the Sichuan Pig Innovation Team of National Modern Agricultural Industry Technology System of China(scsztd-2020-08-11)。

摘　　要：Background Intestinal inflammation is the main risk factor causing intestinal barrier dysfunction and lipopolysaccharide(LPS)can trigger inflammatory responses in various eukaryotic species.Yeast hydrolysate(YH)possesses multibiological effects and is received remarkable attention as a functional ingredient for improving growth performance and promoting health in animals.However,there is still inconclusive on the protective effects of dietary YH supplementation on intestinal barrier of piglets.This study was conducted to investigate the attenuate effects of YH supplementation on inflammatory responses and intestinal barrier injury in piglets challenged with LPS.Methods Twenty-four piglets(with an average body weight of 7.42±0.34 kg)weaned at 21 days of age were randomly assigned to one of two dietary treatments(12 replications with one pig per pen):a basal diet or a basal diet containing YH(5 g/kg).On the 22nd d,6 piglets in each treatment were intraperitoneally injected with LPS at 150μg/kg BW,and the others were injected with the same amount of sterile normal saline.Four hours later,blood samples of each piglet were collected and then piglets were euthanized.Results Dietary YH supplementation increased average daily feed intake and average daily gain(P<0.01),decreased the ratio of feed intake to gain of piglets(P sponse,evidenced by the increase o=0.048).Lipopolysaccharide(LPS)injection induced systemic inflammatory ref serum concentrations of haptoglobin(HP),adrenocorticotropic hormone(ACTH),cortisol,and interleukin-1β(IL-1β).Furthermore,LPS challenge resulted in inflammatory intestinal damage,by up-regulation of the protein or mRNA abundances of tumor necrosis factor-α(TNF-α),IL-1β,toll-like receptors 4(TLR4)and phosphor-nuclear factor-κB-p65(p-NFκB-p65)(P<0.01),and down-regulation of the jejunal villus height,the protein and mRNA abundances of zonula occludens-1(ZO-1)and occludin(OCC;P<0.05)in jejunal mucosa.Dietary YH supplementation decreased the impaired effects of ACTH,cortisol,HP,IL-1βand diam

关 键 词：Inflammatory response Intestinal barrier LIPOPOLYSACCHARIDE PIGLETS Yeast hydrolysate 

分 类 号：S828.5[农业科学—畜牧学]