参芪补肺汤对慢性阻塞性肺疾病大鼠间质性肺炎的作用及机制  

The effect and mechanism of shenqibufei decoction on interstitial pneumonia in COPD rats

作　　者：谌洪俊 邵娜娜 陈昱江 Chen Hongjun;Shao Nana;Chen Yujiang(Center for Preventive Medicine,the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang Guizhou 550002,China;Department of Endocrinology,Traditional Chinese Medicine Hospital of Jinsha County,Bijie Guizhou 551899,China;Department of Pathology,the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang Guizhou 550002,China)

机构地区：[1]贵州中医药大学第一附属医院治未病中心,贵州贵阳550002 [2]金沙县中医院内分泌科,贵州毕节551899 [3]贵州中医药大学第一附属医院病理科,贵州贵阳550002

出　　处：《遵义医科大学学报》2023年第7期652-656,共5页Journal of Zunyi Medical University

基　　金：贵州省科学技术基金资助项目[NO:黔科合LH字(2014)7335]。

摘　　要：目的探讨参芪补肺汤对慢性阻塞性肺疾病大鼠间质性肺炎的治疗作用及其可能的机制。方法将SD大鼠随机分为4组:正常对照组(NC)10只,COPD对照组(CC)10只,正常治疗组(NT)10只,COPD治疗组(CT)10只。通过气管内注入脂多糖及烟熏法制作COPD模型,正常给药组及COPD给药组以参芪补肺汤灌胃治疗;其余两组以等量生理盐水灌胃。4周后取肺组织行HE及Masson染色观察各组大鼠病理改变,通过CD68及CD206免疫组化染色观察巨噬细胞的渗出差异,以Western-blotting检验各组大鼠TGF-β1、Collagen-Ⅳ及Fibronectin的变化。结果COPD大鼠肺组织肺间隔增厚、细胞外基质增多,伴CD68及CD206阳性细胞渗出增多,且TGF-β1、Collagen-Ⅳ、Fibronectin表达增多。经过参芪补肺汤治疗后,COPD治疗组大鼠肺组织病变减轻、CD68及CD206阳性细胞渗出明显减少、纤维化指标表达明显减轻。结论M2巨噬细胞可能参与了COPD大鼠间质性肺炎的发生发展、参芪补肺汤可能通过减轻M2巨噬细胞的渗出而减轻COPD大鼠肺间质纤维化。Objective To study the therapeutic effect of Shenqibufei decoction on interstitial pneumonia in rats with chronic obstructive pulmonary disease(COPD).Methods SD rats were randomly divided into 4 groups:normal control group,COPD control group,normal treated group and COPD treated group,ten rats in each group.The COPD model was prepared by endotracheal lipopolysaccharide and smoking.The normal treated group and COPD treated group were gavaged with Shenqibufei decoction;the other two groups were gavaged with equal amount of normal saline.Four weeks later,rats were killed,and the pathological changes of lung were observed after staining by HE and Masson;the differential exudation of M2 macrophages was analyzed after immunohistochemical staining with CD68 and CD206;and the changes of TGF-β1,Collagen-Ⅳand Fibronectin in each group were tested by Western-blotting.Results Rats with COPD presented septal thickening,increased extracellular matrix,increased exudation of CD68 and CD206 positive cells from perivascular and pulmonary interstitium,and increased expression of TGF-β1,collagen-Ⅳand fibronectin were observed.After treating with Shenqibufei decoction,the lesions in lung were reduced,the exudation of CD68 and CD206 positive cells was significantly reduced,and the expression of fibrosis index was also significantly reduced.Conclusion M2 macrophages were involved in interstitial pneumonia in COPD rats,and Shenqibufei decoction may reduce interstitial fibrosis in COPD rats by alleviating the exudation of M2 macrophages.

关 键 词：慢性阻塞性肺疾病 间质性肺炎 参芪补肺汤 巨噬细胞 

分 类 号：R563.3[医药卫生—呼吸系统]