HPLC法测定芴甲氧羰基-叔丁基-酪氨酸(Fmoc-Tyr(tBu)-OH)有关物质  

Determination of Related Substances in Fmoc-Tyr(tBu)-OH by HPLC

作　　者：程婷[1] 卞从明 刘虎 董世建[1] 朱凯莉 李增礼[1] Cheng Ting;Bian Congming;Liu Hu;Dong Shijian;Zhu Kaili;Li Zengli(Anhui Anke BiotechnologyCo.,Ltd.,Hefei,China)

机构地区：[1]安徽安科生物工程<集团>股份有限公司,合肥安徽

出　　处：《科学技术创新》2023年第20期87-90,共4页Scientific and Technological Innovation

摘　　要：本文建立了定量测定Fmoc-Tyr(tBu)-OH有关物质的高效液相色谱分析方法。该方法以Waters XSelect® CSH^(TM) C18(4.6×250 mm,5μm)柱为色谱柱,以0.05%磷酸-乙腈梯度洗脱,流速1.0 mL·min^(-1),柱温30℃,检测波长220 nm。建立的方法操作简便,专属性强,精密度、准确度良好,可用于Fmoc-Tyr(tBu)-OH有关物质检测。To establish an HPLC method for the determination of related substances in Fmoc-Tyr(tBu)-OH.Waters XSelect® CSH^(TM) C18(4.6×250 mm,5μm)was chosen for analysis.Gradient elution was used to determine the related substances of Fmoc-Tyr(tBu)-OH with 0.05%phosphoric acid and acetonitrile as mobile phase.The flow rate was 1.0 mL·min^(-1) and the column temperature was 30℃.The detection wavelengths were 220 nm.The method is proved to be simple,good precision and high accuracy,and could be used for the determination of related substances in Fmoc-Tyr(tBu)-OH.

关 键 词：合成多肽 起始物料 保护氨基酸 质量控制 高效液相色谱 

分 类 号：O657.72[理学—分析化学]