Genetic variation at the p53 locus of two ecologically divergent Microtus pine voles:identification of molecular markers for species assignment  

作　　者：Ana Sofia QUINA João Paulo TAVANEZ Maria da Luz MATHIAS 

机构地区：[1]CESAM-Centro de Estudos do Ambiente e do Mar,Departamento de Biologia,Universidade de Aveiro,Aveiro,Portugal [2]CESAM-Centro de Estudos do Ambiente e do Mar,Faculdade de Ciências,Universidade de Lisboa,Lisboa,Portugal [3]Departamento de Biologia Animal,Faculdade de Ciências,Universidade de Lisboa,Lisboa,Portugal [4]Global Health and Tropical Medicine(GHTM),Instituto de Higiene e Medicina Tropical(IHMT),Universidade NOVA de Lisboa(UNL),Portugal

出　　处：《Integrative Zoology》2022年第6期1179-1192,共14页整合动物学（英文版）

基　　金：Fundação para a Ciência e a Tecnologia/Ministério da Ciência,Tecnologia e Ensino Superior(FCT/MCTES)for the financial support to CESAM(UIDP/50017/2020+UIDB/50017/2020);through national funds.ASQ and JPT are funded by national funds(OE),through FCT。

摘　　要：The Lusitanian(Microtus lusitanicus)and the Mediterranean(Microtus duodecimcostatus)pine voles are recently diverged sister species endemic of the Iberian Peninsula that can be identified with ecological and morphological characters,but in areas where the 2 species co-occur,species designation may be difficult.Genetic discrimination between M.lusitanicus and M.duodecimcostatus has not been achieved yet possibly because of their estimated re-cent split and an evolutionary history that includes inter-species geneflow.Following our previous observations on exons 5–7 of the p53 gene,here we analyze the potential use of the p53 genomic region as a discrimination marker of these species by extending our analyses to several kb upstream and downstream of the p53 gene and charac-terizing the degree of genetic differentiation in 7 markers within this region.Additionally,we fully sequenced the P53 protein of both species.We observed:(i)generally high differentiation in this region;(ii)M.duodecimcostatus showed in general higher values of nucleotide and haplotype diversities;(iii)the concatenated phylogenetic tree separates the 2 species;(iv)the 2 P53 proteins only differ in 1 amino acid;(v)4 of the markers,2 in p53,one in Atp1b2,and another in Wrap53,contain species-specific genetic variation thus allowing a reliable discrimination between specimens from both species,irrespective of sampling location or introgression status.We provide ad-ditional data on the putative role of p53 in the evolution of these species and present researchers with a fast and cost-effective resource for M.lusitanicus and M.duodecimcostatus identification.

关 键 词：ecological stress genetic variation MICROTUS molecular marker P53 

分 类 号：Q95[生物学—动物学]