Characteristics of the multiple replicon plasmid IncX1-X1 in multidrug-resistant Escherichia coli from Malayan pangolin(Manis javanica)  

作　　者：Fang JI Shelan LIU Xue WANG Jianan ZHAO Jiayue ZHU Jianchun YANG Chenglin ZHANG Zhongxin JIA Ruili ZHAO Guocheng HU Jing WANG Jianhua QIN Gang LI Bin WU Chengmin WANG 

机构地区：[1]Guangdong Key Laboratory of Animal Conservation and Resource Utilization,Guangdong Public Laboratory of Wild Animal Conservation and Utilization,Institute of Zoology,Guangdong Academy of Science,Guangzhou,China [2]Department of Infectious Diseases,Zhejiang Center of Disease Control and Prevention,Hangzhou,Zhejiang,China [3]School of Bioengineering,East China University of Science and Technology,Shanghai,China [4]Beijing Key Laboratory of Captive Wildlife Technologies,Beijing Zoo,Beijing,China [5]College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin,China [6]South China Institute of Environmental Sciences,Ministry of Ecology and Environment,Guangzhou,China [7]Department of Infectious Diseases,Hangzhou Center of Disease Control and Prevention,Hangzhou,China [8]College of Veterinary Medicine,Agricultural University of Hebei,Baoding,China

出　　处：《Integrative Zoology》2023年第2期289-298,共10页整合动物学（英文版）

基　　金：supported by the Introduction of Leading Talents Program of the Guangdong Academy of Sciences(No.2016GDASRC-0205);the Open project of Beijing Key Laboratory of captive wildlife technology in Beijing Zoo(ZDK202105).

摘　　要：Potential zoonotic pathogens may be transmitted from wildlife to humans through the illegal wild meat trade,which has become a pressing issue.However,research on the antimicrobial resistance genes(ARGs)of Malayan pangolin(Manis javanica)intestinal bacteria is limited.Here,multidrug-resistant Escherichia coli M172-1(ST354)isolated from Malayan pangolin feces in 2019 was found to be resistant to 13 antibiotics.BGWAS analysis revealed 4 plas-mids,namely,pM172-1.1,pM172-1.2,pM172-1.3,and pM172-1.4,in the isolate.The pM172-1.2,pM172-1.3,and pM172-1.4 plasmids carried ARGs,namely,IncHI2-HI2A,IncX1-X1,and IncX1,respectively.pM172-1.3 and pM172-1.4 contained intact IntI1 integrons(Is26/IntI1/arr2/cmlA5/blaOXA-10/ant(3′′)-IIA/dfrA14/Is26).No-tably,pM172-1.3 resulted from the fusion of 2 pM172-1.4 copies and carried many more ARGs.In addition to pM172-1.3 from the same host,other drug-resistant bacteria(E.coli M159-1(ST48),E.coli S171-1(ST206),and Klebsiella pneumoniae S174-1(ST2354))in the same Malayan pangolin fecal samples also carried 3 plasmids with 100%gene coverage of pM172-1.4 and 99.98%identity.Therefore,ARGs in IncX1 might spread in the intestinalflora of Malayan pangolin and between species via the illegal food chain,posing a potential threat to public health and safety.

关 键 词：ARG Manis javanica multiple-replicon plasmid IncX1-X1 plasmid IncX1 

分 类 号：R37[医药卫生—病原生物学]