Simultaneous analysis of the intestinal parasites and diet through eDNA metabarcoding  

作　　者：Xabier CABODEVILLA Benjamín Juan GÓMEZ-MOLINER Naiara ABAD María JoséMADEIRA 

机构地区：[1]Department of Zoology and Animal Cell Biology,Faculty of Pharmacy,University of the Basque Country(UPV/EHU),Alava,Spain [2]Instituto de Investigación en Recursos Cinegéticos(IREC)(CSIC-UCLM-JCCM),Ciudad Real,Spain

出　　处：《Integrative Zoology》2023年第3期399-413,共15页整合动物学（英文版）

基　　金：Xabier Cabodevilla was supported by a PhD grant,financed by the Basque Country Government(Grants no.PRE_2018_2_0273);a contribution to project Sistemática,Biogeografía,Ecología del comportamiento y Evolución(IT1163-19);funded by Basque Country Government;Additional funds for this study were provided by the project 201630E096 funded by CSIC.

摘　　要：Agricultural expansion and intensification are having a huge impact on plant and arthropod diversity and abun-dance,affecting food availability for farmland birds.Difficult food access,in turn,can lead to immunosuppression and a higher incidence of parasites.In the studies designed to examine changes in the diet of birds and their par-asites,metabarcoding is proving particularly useful.This technique requires mini-barcodes capable of amplifying the DNA of target organisms from fecal environmental DNA.To help to understand the impact of agricultural expansion on biodiversity,this study sought to design and identify mini-barcodes that might simultaneously as-sess diet and intestinal parasites from the feces of farmland birds.The capacity to identify diet and parasites of 2 existing and 3 newly developed mini-barcodes was tested“in silico”in relation to the behavior of a reference eukaryotic barcode.Among the newly designed mini-barcodes,MiniB18S_81 showed the higher taxonomic cover-age of eukaryotic taxa and a greater amplification and identification capacity for diet and parasite taxa.Moreover,when it was tested on fecal samples from 5 different steppe bird species,MiniB18S_81 showed high taxonomic resolution of the most relevant diet and parasite phyla,Arthropoda,Nematoda,Platyhelminthes,and Apicomplexa at the order level.Thus,the mini-barcode developed emerges as an excellent tool to simultaneously provide detailed information regarding the diet and parasites of birds,essential for conservation and management.

关 键 词：BIOMONITORING broad-spectrum primers eukaryotic mini-barcodes fecal eDNA farmland birds PARASITOLOGY 

分 类 号：R53[医药卫生—内科学]