Effects of water-borne copper on the gills and hepatopancreas of Macrobrachium rosenbergii  

作　　者：Na LI Qiang MA Jian YANG Yunlong ZHAO Jean-Claude BROCHON 

机构地区：[1]School of Life Science,East China Normal University,Shanghai,China [2]Key Laboratory of Ecological Environment and Resources of Inland Fisheries,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi,China [3]National Research Center,Laboratory of Biotechnology and Application Genetic Pharmacology,Ecole Normale Supérieure Cachan,Cachan cedex,France

出　　处：《Integrative Zoology》2008年第4期299-310,共12页整合动物学（英文版）

基　　金：supported by the Ministry of Personnel(Scientific Research Foundation for Returned Overseas Chinese Scholars,2004,Project No.2-105053);the Chinese Academy of Fishery Sciences for Chief Scientists(Project No.1-105011);the Science and Technology Department of Shanghai(Project No.07DZ12038);the Cultivation Fund of The Key Scientific and Technical Innovation Innovation Project,the Ministry of Education of China under contract(Project No.704023).

摘　　要：We focused on elucidating the toxic effects of water-borne copper on the giant freshwater prawn Macrobrachium rosenbergii.After seven days of exposure to copper(Cu2+)at concentrations ranging from 0.01 mg L–1 to 0.5 mg L–1,three isozymes,malate dehydrogenase,alkaline phosphatase and esterase,were analyzed and compared using polyacrylamide electrophoresis(PAGE)and biochemical staining.The results indicated that the electrophoretic patterns of the isozymes showed a copper-concentration-related difference.Low doses of copper stimulated strong expression of the three isozymes.Electrophoretic patterns of malate dehydrogenase and alkaline phosphatase gradually became weaker or even lost as the level of copper increased.In contrast,esterase patterns exhibited an increased molecular heterogeneity at higher copper concentrations.A transmission electron microscope was used to study ultrastructure differences in the gills and hepatopancreas of M.rosenbergii,and the results showed significant structural damage at increased levels of copper compared with the control group.The basement mem-branes and mitochondira in the gills were seriously damaged,the cuticle electron density distribution was not homogeneous,and an infolded basement membrane,circularized nucleus,disintegrated nuclear membrane,and decreased mitochondria number and size were observed in the gills.Similarly,flowing out of karyoplasms,partly falling microvilli,decreased mitochondrion,partly disappeared mitochondrial cristae,and a thinned matrix were observed in the hepatopancreas.These findings indicate that exposure to elevated copper levels might damage the ultrastructure of the gills and hepatopancreas of M.rosenbergii and might further weaken their normal physical activities.Isozymes were quite sensitive to environmental stress and changes in isozyme elctrophoretic patterns might be effective biomarkers of environmental contamination.

关 键 词：COPPER GILLS HEPATOPANCREAS ISOZYMES Macrobrachium rosenbergii polyacrylamide electrophoresis. 

分 类 号：TG1[金属学及工艺—金属学]