智能聪配施壳寡糖和褐藻寡糖对烟草生长和病毒抗性的影响  

Effects of ZNC combined with chitosan oligosaccharide and alginate oligosaccharides on the growth and viral disease resistance in tobacco

作　　者：张晓英 王胜 王洪凤 王珊珊 王庆彬 石磊[1] ZHANG Xiaoying;WANG Sheng;WANG Hongfeng;WANG Shanshan;WANG Qingbin;SHI Lei(Microelement Research Centre,Huazhong Agricultural University,Wuhan 430070,China;Shandong Pengbo Biotechnology Co.,Ltd.,Tai’an 271000,China)

机构地区：[1]华中农业大学微量元素研究中心,武汉430070 [2]山东蓬勃生物科技有限公司,泰安271000

出　　处：《植物保护》2023年第4期41-46,74,共7页Plant Protection

基　　金：国家重点研发计划(2023YFD1700204);山东省重大科技创新工程(2019JZZY020608)。

摘　　要：智能聪(ZNC)是宛氏拟青霉Paecilomyces variotii的提取物,前期研究证明其具有促进植物生长,提高作物产量、品质和抗病的能力。为了进一步提高产品应用效果,本研究将智能聪分别与壳寡糖和褐藻寡糖复配,评价其对烟草生长和病毒病抗性的影响。结果表明,智能聪与壳寡糖或褐藻寡糖复配后的促生效果和对马铃薯X病毒(potato virus X,PVX)的抑制作用优于单剂,其中促生效果最佳的为0.05μg/mL智能聪与12μg/mL褐藻寡糖复配,处理后烟草株高、根长、侧根数、鲜重和干重分别比单施褐藻寡糖增加26.31%、20.41%、23.20%、23.60%和31.03%;对PVX病毒抑制作用最好的是0.05μg/mL智能聪与30μg/mL壳寡糖复配,对PVX抑制率为71.26%。研究结果可为防治烟草花叶病的药剂复配选择提供依据。The immune inducer Zhinengcong(ZNC)is an extract from Paecilomyces variotii.Previous studies showed that ZNC had the ability to promote plant growth and increase plant yield and disease resistance.To further improve its efficacy in the field,ZNC was combined with chitosan oligosaccharide and alginate oligosaccharides to study their synergistic effects on tobacco growth promotion and virus resistance.The results showed that the combination of ZNC with chitosan oligosaccharide and alginate oligosaccharides led to better growth performance and higher resistance to potato virus X(PVX)as compared with single agents.The best combination for the growth of tobacco was the mixture of 0.05μg/mL ZNC and 12μg/mL alginate oligosaccharides,which significantly increased plant height,root length,root number,fresh weight and dry weight by 26.31%,20.41%,23.20%,23.60%and 31.03%,respectively.The best combination for inhibiting PVX was the mixture of 0.05μg/mL ZNC and 30μg/mL chitosan oligosaccharide,which significantly inhibited PVX by 71.26%.These results provide a basis for the selection of compound pesticides for controlling tobacco mosaic caused by PVX in the field.

关 键 词：马铃薯X病毒 智能聪 促生 抗病 壳寡糖 褐藻寡糖 

分 类 号：S432.4[农业科学—植物病理学]