基于原噬菌体类型的广西柑橘黄龙病菌种群分析  

Population of‘Candidatus Liberibacter asiaticus’in Guangxi based on the prophage types

作　　者：李战彪[1] 林林 陈锦清 谢慧婷[1] 崔丽贤[1] 莫翠萍 刘丽辉 秦碧霞[1] 蔡健和[1] LI Zhanbiao;LIN Lin;CHEN Jinqing;XIE Huiting;CUI Lixian;MO Cuiping;LIU Lihui;QIN Bixia;CAI Jianhe(Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pest,Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs,Plant Protection Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Guangxi Academy of Agricultural Sciences Yulin Branch,Yulin Academy of Agricultural Sciences,Yulin 537000,China)

机构地区：[1]广西壮族自治区农业科学院植物保护研究所,农业农村部华南果蔬绿色防控重点实验室,广西作物病虫害生物学重点实验室,南宁530007 [2]广西壮族自治区农业科学院玉林分院,玉林市农业科学院,玉林537000

出　　处：《植物保护》2023年第4期312-317,333,共7页Plant Protection

基　　金：广西创新驱动发展专项资金(桂科AA18118046-5,桂科AA18118046-8);广西农业科学院基本科研业务专项(桂农科2021YT071);广西柑橘创新团队桂东站(nycytxgxcxtd-2021-05-04)。

摘　　要：2016年-2021年,从广西各柑橘产区采集叶片斑驳、黄化等疑似黄龙病症状的柑橘样品,利用黄龙病特异引物和基于原噬菌体类型的特异引物对样品进行鉴定及分析,结果显示,所采集的3293份样品中有856份样品为黄龙病阳性样品,阳性样品的检出率为25.99%,阳性样品在广西的14个地市均有分布。基于原噬菌体类型的黄龙病菌种群分析发现,广西存在7种类型的菌株,分别是type 1菌株、type 2菌株、type 3菌株、type 1+type 2菌株、type 2+type 3菌株、type 1+type 2+type 3菌株及未检出任何类型菌株(none type),其中,type 2类型原噬菌体菌株为优势种群。During 2016-2021,citrus leaf samples with mottle,yellow vein symptoms were collected from the citrus growing region in Guangxi.Huanglongbing(HLB)-specific primers and prophage type-specific primers were used for detection and analysis.The results showed that 856 out of the total 3293 samples were detected as HLB positive samples by PCR using HLB-specific primers,with detection rate of 25.99%,and HLB could detected from all 14 cites in Guangxi.The population of‘Candidatus Liberibacter asiaticus(CLas)’in Guangxi was also analyzed based on the prophage.Seven combinations of CLas prophages were observed in the CLas from all the tested samples,including single type-1 CLas,single type-2 CLas,single type-3 CLas,type1+type2 CLas,type2+type3 CLas,type1+type2+type3 CLas and none type,among them,type-2 CLas prophage was the predominant population in Guangxi.

关 键 词：广西 柑橘 柑橘黄龙病 原噬菌体 种群类型 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]